Addgene

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3XFlag pCMV5-TOPO TAZ (∂393)

Plasmid

#24812

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-393. (No PDZ binding…

Promoter

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLX_TRC311 ETV4-S

Plasmid

#74983

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 )

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-M2-PP1Cbeta

Plasmid

#31677

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98932

Purpose

Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4938

Plasmid

#107527

Purpose

Expresses Fn crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLX307 DEDD

Plasmid

#117744

Purpose

Open reading frame vector encoding DEDD

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

DEDD1 (DEDD Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-1x

Plasmid

#124685

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shETV1-A

Plasmid

#74973

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM5-mDPYD

Plasmid

#61614

Purpose

The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidine dehydrogenase (Dpyd Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-TEVsite-human Glomulin

Plasmid

#52292

Purpose

bacterial expression of human Glomulin

Depositor

Brenda Schulman

Article

Duda et al Mol Cell. 2012 Aug 10;47(3):371-82. doi:

Insert

Glomulin (GLMN Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98931

Purpose

Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 5A short

Plasmid

#37484

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 5A (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform C (missing N-term 58aa), 536aa, S150A T15…

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-N-terminus(1-111)

Plasmid

#64671

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

containing only N-terminus amino acids 1 to 111

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1 shETV1-B

Plasmid

#74974

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgKmt2d-sg3

Plasmid

#192167

Purpose

Lenti-sgKmt2d-sg3

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTE4560

Plasmid

#107526

Purpose

Expresses As crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX307 ARHGAP30

Plasmid

#117738

Purpose

Open reading frame vector encoding ARHGAP30

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

ARHGAP30 (ARHGAP30 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge