We narrowed to 27,276 results for: ANT
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Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…MutationAspartic Acid 242 (in Dennis et al.) changed to A…Available SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD59-T2A-GFP
Plasmid#205460Purposelentiviral plasmid for expression of human CD59DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLI1_pcDNA6.2/EmGFP-Bsd
Plasmid#176968PurposeMammalian expression vector encoding GLI1 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRC8400-SARS-CoV-2-RBD-SD1-B.1.1.529-AVI
Plasmid#181704PurposeExpresses SARS-CoV-2 RBD-SD1 domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tagDepositorInsertSARS-CoV-2-S2P-B.1.1.529 (S )
TagsAVI, HRV3C, and scFcExpressionMammalianMutationG339D, S371L, S373P, S375F, K417N, N440K, G446S, …Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
Plasmid#176059PurposeExpressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
Tags3xFLAGExpressionMammalianMutationR124A and K125APromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
Tags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C
Plasmid#171247PurposeMammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
TagsFlagExpressionMammalianMutationAR-Trp741Cys (AR-W741C)PromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HLA-cmyc-optiT1R3 a21-852
Plasmid#113948Purposemammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 21-852PromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Human, Homo sapiens)
UseAAVTagsmCherryExpressionMammalianPromoterHuman UbCAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA
Plasmid#104984PurposeExpresses ST-Renilla reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-KD-mAbl
Plasmid#69028PurposeRetorviral vector encoding Kinase Defective mouse c-AblDepositorInsertmouse c-Abl Type VI - kinase defective
UseRetroviralTagsFLAG tag inserted at Internal SalI site (2934 bp)ExpressionMammalianMutationThe KD (kinase defective) mutation is at Lysine 2…Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-FOXO 3A
Plasmid#52983Purposeinsect expression of FOXO triple mutantDepositorInsertFOXO (foxo Fly)
Tags3x HAExpressionInsectMutation3A, triple mutant, T44A S190A S259APromoterHsp70Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
P12-bio
Plasmid#47725PurposeExpresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised P12
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio
Plasmid#47786PurposeExpresses enzymatically monobiotinylated full-length RAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RAMA
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
scribble cDNA
Plasmid#42064DepositorInsertscribble (scrib Fly)
Available SinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R514G-YFP
Plasmid#29616DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only