We narrowed to 13,211 results for: CAR

1. pET28a LZ LED 4CA-FLAG

   Plasmid

   #199681

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED 4CA (GPR155 Human)
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   C595.604.629.638 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a LZ LED Y551A-FLAG

   Plasmid

   #199680

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED Y551A (GPR155 Human)
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Y551 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-Bnip3-T181A

   Plasmid

   #197556

   Purpose

   Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)

   Depositor

   Joseph Gordon

   Article

   Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.

   Insert

   BCL2 Interacting Protein 3 (Bnip3 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Threonine 181 changed to alanine

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLED.RAB

   Plasmid

   #193015

   Purpose

   Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W

   Plasmid

   #163147

   Purpose

   Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   CHAT (CHAT Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

   Plasmid

   #163175

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

   Plasmid

   #163174

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

   Plasmid

   #187647

   Purpose

   Encodes gRNA to correct the SFTPC I73T mutation

   Depositor

   Darrell Kotton

   Article

   Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

   Insert

   I73T gRNA
   

   Use

   CRISPR

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

   Plasmid

   #161924

   Purpose

   To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting RDH11 exon 2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEXSISERS_NLuc_FRT-PuroR

    Plasmid

    #177865

    Purpose

    Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    NLuc-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEXSISERS_BSD_FRT-PuroR

    Plasmid

    #177867

    Purpose

    Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    BSD-based EXSISERS
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    OLLAS

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEXSISERS_SurfaceHaloTag_FRT-PuroR

    Plasmid

    #177869

    Purpose

    Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    Surface-HaloTag-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Expression

    Mammalian

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid

    #181872

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    

    Use

    AAV and Adenoviral

    Tags

    HA, T2A, and myc

    Expression

    Mammalian

    Mutation

    includes a large portion of the Slc8b1 3'UTR…

    Promoter

    synthetic hybrid CAG promoter

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_S.V5.FLAG_NGFR

    Plasmid

    #158337

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.FLAG

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_S.V5.NWS_NGFR

    Plasmid

    #158338

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.NWS

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO_S.V5.AU1_NGFR

    Plasmid

    #158340

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.AU1

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO_S.V5.VSVg_NGFR

    Plasmid

    #158341

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_Ollas.V5.FLAG_NGFR

    Plasmid

    #158316

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.V5.FLAG

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_Ollas.V5.NWS_NGFR

    Plasmid

    #158317

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.V5.NWS

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_Ollas.V5.HA_NGFR

    Plasmid

    #158318

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.V5.HA

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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