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pCK025

Plasmid

#105415

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain and regulatory sequences (estrogen receptor)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_SOX5

Plasmid

#86292

Purpose

Donor vector for 3' FLAG tag of human SOX5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SOX5 homology arms (SOX5 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_105_Venus_wt

Plasmid

#39818

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
hopscotch

Plasmid

#37284

Depositor

Norbert Perrimon

Article

Binari et al Genes Dev. 1994 Feb 1;8(3):300-12.

Insert

hopscotch (hop Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pFETCh_ZNF644

Plasmid

#86264

Purpose

Donor vector for 3' FLAG tag of human ZNF644

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF644 homology arms (ZNF644 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SOX5_1

Plasmid

#86357

Purpose

Encodes gRNA for 3' target of human SOX5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SOX5 (SOX5 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Tags

MBP

Expression

Bacterial

Mutation

K196R

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
EKAR2G_design2_mTFP_227_Venus_173

Plasmid

#40016

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_227_Venus_229

Plasmid

#39837

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-FERM10

Plasmid

#59126

Purpose

FAK fret control sensor without kinase domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design2_mTFP_wt_Venus_173

Plasmid

#39996

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design2_mTFP_105_Venus_173

Plasmid

#40001

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design2_mTFP_159_Venus_173

Plasmid

#40006

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design2_mTFP_227_Venus_229

Plasmid

#40018

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
EKAR2G_design2_mTFP_175_Venus_229

Plasmid

#40013

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-10FNIII(x4)-DocI

Plasmid

#58712

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

10FNIII(x4)-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design2_mTFP_105_Venus_157

Plasmid

#40000

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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unc5bCD4d3+4-bio-linker-His

Plasmid

#36150

Purpose

EXPRESs plasmid for Zebrafish Receptors encoding zebrafish unc5b (netrin-1a) with rat CD4d3+4-bio-linker-His tag

Depositor

Gavin Wright

Article

Sollner et al Genome Biol. 2009;10(9):R99. Epub 2009 S

Insert

unc5b (unc5b Zebrafish)

Tags

His tag, biotinylation peptide, and ratCD4d3+4

Expression

Mammalian

Mutation

L2V

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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