162,999 results

1. APEX2-GBP

   Plasmid

   #67651

   Purpose

   Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-hMYOD1 (#794)

   Plasmid

   #78329

   Purpose

   Overexpression of human MYOD1 ORF with a N-terminal FLAG tag

   Depositor

   Matthew Alexander, Louis Kunkel

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CamKIIa-jGCaMP8f-WPRE

   Plasmid

   #176750

   Purpose

   AAV-mediated expression of GCaMP8f under the CamKIIa promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIalpha

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-hERG1a

   Plasmid

   #53052

   Purpose

   Expresses alpha subunit of hERG1a K channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-CRM197-4xDQNAT

   Plasmid

   #128403

   Purpose

   Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Diphtheria toxin
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating G52E mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE792

   Plasmid

   #167300

   Purpose

   Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 IRF7(5’UTR)-FF

   Plasmid

   #85490

   Purpose

   Firefly luciferase under the control of IRF7 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of IRF7 (Irf7 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215327

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   MUC5AC Promoter
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MUC5AC

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-Tev-UbE1

    Plasmid

    #186804

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE1 (UBA1 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEAK-Flag/INPP4B

    Plasmid

    #24324

    Depositor

    Lewis Cantley

    Article

    Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

    Insert

    Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid

    #162280

    Purpose

    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN8A Variant 1, stabilized (SCN8A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    Modified human HSPA5 intron 4 inserted after bp23…

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CuO-LacI-NLS–SNAPf

    Plasmid

    #227368

    Purpose

    Lentiviral expression of LacI–SNAP

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    LacI NLS SNAPf
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_S5E2_Chrimson_tdTomato

    Plasmid

    #219432

    Purpose

    AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

    Depositor

    Gordon Fishell

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    Chrimson-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TrpC5 scFv [N67/15]

    Plasmid

    #190564

    Purpose

    Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

    Plasmid

    #74929

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-BFP-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. CVB3 IRES

    Plasmid

    #189968

    Purpose

    Part 2 plasmid to introduce the CVB3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    CVB3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1 puro p53 shRNA 1

    Plasmid

    #10671

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120395

    Purpose

    pAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Scrambled shRNA
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-hygro CDK4 R24C

    Plasmid

    #11254

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    R24C

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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