161,256 results

1. pFUW-TetO-ETV6

   Plasmid

   #131594

   Purpose

   doxycycline-inducible expression of human ETV6 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   ETV6 (ETV6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(Hygro) TagBFP-hNucleolin

   Plasmid

   #182592

   Purpose

   expresses bfp-hNucleolin in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hNucleolin (NCL Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCN-EF2132tet

   Plasmid

   #107191

   Purpose

   Expresses EF2132 recombinase for performing recombineering in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   EF2132
   tetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p23

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Has Service

   AAV6

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-C1

   Plasmid

   #53975

   Purpose

   Localization: C1 Cloning Vector, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMXs-IP-PEX3-EGFP

   Plasmid

   #228142

   Purpose

   Expresses PEX3-GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   PEX3 (PEX3 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_eA3A-NG-nCas9

   Plasmid

   #163536

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. RabV CVS-N2c(deltaG)-mCherry

   Plasmid

   #73464

   Purpose

   Expresses mCherry for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   mCherry
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM-GFP

    Plasmid

    #21213

    Depositor

    Tobias Meyer

    Article

    Teruel et al J Neurosci Methods. 1999 Oct 30. 93(1):3

    Insert

    PM-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PM targetting sequence is Lyn (MGCIKSKRKD) (Lab …

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

    Plasmid

    #99608

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    iCRE
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Use

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid

    #190597

    Purpose

    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    EGFP - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-M2-TLR2

    Plasmid

    #12291

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I and H202Y; signal peptide (aa1-23) replaced …

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pMZ0002

    Plasmid

    #102239

    Purpose

    Expresses Chinese hamster NSF in bacteria

    Depositor

    Axel Brunger

    Article

    Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

    Insert

    N-ethylmaleimide sensitive factor
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUBC_hsPUM1-mCherry

    Plasmid

    #107607

    Purpose

    Transient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoter

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    hsPUM1-mCherry
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human UBC

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. bactin:GFP-LAP2b

    Plasmid

    #105980

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    LAP2b
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGR481

    Plasmid

    #213783

    Purpose

    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

    Depositor

    Chang Liu

    Insert

    ura3*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpRY-YE1-BE4max

    Plasmid

    #198554

    Purpose

    Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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