We narrowed to 18,496 results for: Met

1. pSprayerHE

   Plasmid

   #165150

   Purpose

   Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Insert

   BASU-GAL4
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSNK

   Plasmid

   #135193

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBait

   Plasmid

   #165146

   Purpose

   Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_nLightG

   Plasmid

   #217656

   Purpose

   Expresses norepinephrine indicator nLightG under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightG
   

   Expression

   Mammalian

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-HA-UFL1

   Plasmid

   #139860

   Purpose

   Lentiviral expression of HA-tagged human UFL1

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   UFL1 (UFL1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28c(+)-YTHDF3

   Plasmid

   #102276

   Purpose

   For bacterial expression of His-tagged human YTHDF3

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF3 (YTHDF3 Human)
   

   Tags

   6X HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mVenus-IQ6 WT

   Plasmid

   #198201

   Purpose

   FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   MYO5A IQ domain (Myo5a Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Promoter

   FCP and U6

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRVL-3

   Plasmid

   #104581

   Purpose

   Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
   

   Expression

   Mammalian

   Mutation

   L234A/L235E/G237A and E318A/K320A/K322A

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. esmGFP-C10-pVTCB052

    Plasmid

    #231303

    Purpose

    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

    Depositor

    Alexander Rives

    Insert

    esmGFP-C10
    

    Expression

    Bacterial

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR-mTagBFP2

    Plasmid

    #228596

    Purpose

    Mammalian expression of mTagBFP2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR-mRuby3

    Plasmid

    #228609

    Purpose

    Mammalian expression of mRuby3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mRuby3
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS_cAMPFIRE-H

    Plasmid

    #182285

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SiC-V1-Scr

    Plasmid

    #133042

    Purpose

    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMK074: CMV QuasAr2-TS-GCaMP6f

    Plasmid

    #72303

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TAG-ERN

    Plasmid

    #80476

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-CRY2-5ptaseOCRL

    Plasmid

    #66836

    Purpose

    Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    5ptaseOCRL (OCRL Human)
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSprayer

    Plasmid

    #165148

    Purpose

    Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    BASU-GAL4
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS614_pTetR-P2A-BFPnls/sgNS

    Plasmid

    #108650

    Purpose

    Non-specific Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Non-specific sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PL-SIN-PGK-EiP

    Plasmid

    #21312

    Purpose

    Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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