169,365 results
-
-
pUltra-U6-gRNAs1-5
Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc
Plasmid#16539Purposeluciferase reporter plasmid with very low basal activityDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
Plasmid#207861PurposeAAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
UseAAVMutationSee ManuscriptPromoterCbhAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 AIF-mScarlet
Plasmid#129576PurposeExpression of mScarlet fused AIFDepositorAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2A-EKAREV-NLS
Plasmid#173856PurposeEncoding EKAREV-NLS.DepositorInsertEKAREV-NLS
ExpressionMammalianAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN-I-GFP
Plasmid#1736PurposeThis retroviral vector has an IRES-GFP downstream of the cloning sitesDepositorInsertIRES-GFP
UseRetroviralAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
3XMEF2-luc
Plasmid#32967PurposeReporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoterDepositorInsertMEF2 binding sites (MEF2A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterMEF2Available SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-h53BP1 (siRNA resistant)
Plasmid#110301PurposeMammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)DepositorInsertp53-binding protein 1 (TP53BP1 Human)
TagsEGFPExpressionMammalianMutationGAACGAGGA to GAGCGGGGCAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ-mgU2-47-Mango II
Plasmid#127586PurposeExpresses mgU2-47-Mango II scaRNA from a mU6 promoterDepositorInsertmgU2-47 scaRNA with Mango II tag
ExpressionMammalianPromotermodified U6Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AcGFP1-ORP9-PH
Plasmid#214266PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.VSVg_mCherry-NLS
Plasmid#178214PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.VSVg and Nuclear Localization SignalPromotereF1aAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR
Plasmid#131614PurposeExpresses NeoR and dsRed.DepositorInsertsNeoR
dsRed
ExpressionInsectPromoterOpie2 and hr5Ie1Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA
Plasmid#184635PurposeExpresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B WT
Plasmid#175025PurposeExpresses WIPI2B fused to Halo in mammalian cellsDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
SlugMyc_pcDNA3
Plasmid#31698DepositorAvailable SinceOct. 7, 2011AvailabilityAcademic Institutions and Nonprofits only
