162,999 results

1. pEGFP-C1-A20

   Plasmid

   #22141

   Depositor

   Yihong Ye

   Article

   Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

   Insert

   A20 (TNFAIP3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197027

   Purpose

   Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enRhCas12f1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L270R

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163076

   Purpose

   Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA407

   Plasmid

   #85970

   Purpose

   UPRE-mCh-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   mCherry
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

   Plasmid

   #193942

   Purpose

   a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

   Depositor

   Greg Delgoffe

   Article

   Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

   Insert

   heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp6-mNeongreen

   Plasmid

   #182388

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK-2-Smad2-3'UTR

   Plasmid

   #61775

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Smad2

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Smad2 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. TFORF3343

   Plasmid

   #144819

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RARB (RARB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagRFP-T-Vimentin-7

   Plasmid

   #58029

   Purpose

   Localization: Intermediate Filaments, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   NM_003380.3 (VIM Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMSC168

    Plasmid

    #216350

    Purpose

    xhNup35-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EpsilonCOP EGFP

    Plasmid

    #128151

    Purpose

    Expression of epsilon COP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    epsilon COP (COPE Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K3753R

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Ash2L full

    Plasmid

    #15548

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R622H (Please see depositor comments below)

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MscL(G22S)61-cpGFP-ERexp

    Plasmid

    #195311

    Purpose

    Fluorescent reporter/biosensor for membrane tension

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)61-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge