We narrowed to 15,978 results for: grna

1. pCFD5-NS

   Plasmid

   #149546

   Purpose

   Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG2

   Plasmid

   #162603

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330S-7

   Plasmid

   #58783

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB3405

   Plasmid

   #106166

   Purpose

   EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRT2B-GO2

   Plasmid

   #136909

   Purpose

   Lentivirus expressing GO2 sgRNA

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   sgGO2
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   hU6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-U6insert

   Plasmid

   #104536

   Purpose

   A piggybac vector with a U6 promoter that allows cloning of guide RNAs

   Depositor

   George Church

   Article

   Kalhor et al Science. 2018 Aug 9. pii: science.aat980

   Type

   Empty backbone

   Use

   CRISPR, Mouse Targeting, and Synthetic Biology ; …

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-Hyg

   Plasmid

   #158708

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Expression

   Bacterial

   Promoter

   Hsp60

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330A-1x3

   Plasmid

   #58767

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pltetO1

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ146

    Plasmid

    #69298

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. JME4472

    Plasmid

    #129658

    Purpose

    URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRGEB33

    Plasmid

    #158151

    Purpose

    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRGE31

    Plasmid

    #50929

    Purpose

    Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PLKO.1-USP10-3'UTR

    Plasmid

    #136056

    Purpose

    USP10 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTCTCTTTAGTGGCTCTTT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    USP10 (USP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI) promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. siSlug3

    Plasmid

    #10905

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    siSlug (SNAI2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Stuffer

    Plasmid

    #106248

    Purpose

    Destination vector for U6::gRNA expression cassette

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Type

    Empty backbone

    Use

    AAV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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