We narrowed to 16,087 results for: grna

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p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-mCherry-shPtbp1

Plasmid

#178589

Purpose

Expression of mCherry and a shRNA against Ptbp1 under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Ptbp1

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMM733

Plasmid

#68898

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-mCherry-shLuci

Plasmid

#178588

Purpose

Expression of mCherry and a shRNA against luciferase under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Luciferase

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCA0.421

Plasmid

#203950

Purpose

Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad4

Plasmid

#15724

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pYPQ145

Plasmid

#69297

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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shNg pA_RC3J1_CAGW

Plasmid

#92155

Purpose

Bicistronic AAV construct encoding GFP control and shRNA targeting Ng

Depositor

Weifeng Xu

Article

Han et al Cell Rep. 2017 Jan 24;18(4):892-904. doi

Insert

shRNA targeting Ng (NRGN Human)

Use

AAV

Expression

Mammalian

Promoter

H1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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OgeuIscB ωRNA-v13

Plasmid

#219634

Purpose

human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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p8389 LentiCRISPRv2 Neo sgNT-1

Plasmid

#221649

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-1

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_crU626

Plasmid

#191776

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ141B-ge4.1

Plasmid

#205144

Purpose

Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Promoter

AtU3

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLB2 Ubi-FLIP FF

Plasmid

#19753

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, Lentiviral, Mouse Targeting, and RNAi

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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ZO-1 shRNA 2072

Plasmid

#37207

Depositor

James Anderson, Alan Fanning

Article

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-1 shRNA (TJP1 canine)

Use

RNAi

Expression

Mammalian

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only