We narrowed to 12,292 results for: BASE

1. AAV-AID-N51A-CBE N

   Plasmid

   #227421

   Purpose

   expresses the N-terminal of AID-N51A-CBE

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   AAV-AID-N51A-CBE N-terminal (AICDA Human)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-xFNLS-P2A-Puro

   Plasmid

   #110872

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Use

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-FNLS-PGK-Puro

   Plasmid

   #110844

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cbh_v5 AAV-saCBE C-terminal

   Plasmid

   #137183

   Purpose

   AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-saCBE C-terminal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPACE-VRQR (pRZ5137)

   Plasmid

   #140245

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_VRQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, VRQR mutations in SpCas9, D10A in …

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.17-d

   Plasmid

   #136299

   Purpose

   expresses ABE8.17-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-xBE3-P2A-Puro

    Plasmid

    #110870

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSRW1JZ

    Plasmid

    #129528

    Purpose

    C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

    Depositor

    Noelle L'Etoile

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    FlincG3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178827

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RANLS-IETD-BNES

    Plasmid

    #60972

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. ABE8.8-d

    Plasmid

    #136295

    Purpose

    expresses ABE8.8-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. nCas9-UGI control for Target-AID (pRZ1047)

    Plasmid

    #131299

    Purpose

    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_hU6miR-G12-E

    Plasmid

    #25757

    Purpose

    Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 12 miR-shRNA (Gna12 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ABE8.13-d

    Plasmid

    #136297

    Purpose

    expresses ABE8.13-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEN_TmiR_G12-E

    Plasmid

    #25761

    Purpose

    Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 12 miR-shRNA (Gna12 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hyABE

    Plasmid

    #196096

    Purpose

    Expresses hyABE

    Depositor

    Xiahui Zhang

    Article

    Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

    Insert

    hyABE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid

    #235303

    Purpose

    Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235306

    Purpose

    Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEN_hU6miR-Gb2-K

    Plasmid

    #25759

    Purpose

    Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Gb2 miR-shRNA (Gnb2 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

    Plasmid

    #189643

    Purpose

    16S rRNA Right_G1397C_ABE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox and TadA 8e
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRPS5A-PTP-3Xflag-16S rRNA Left_G1397N

    Plasmid

    #189641

    Purpose

    16S rRNA Left_G1397N

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psfaRb-Tn5

    Plasmid

    #183638

    Purpose

    Expresses anti-rabbit nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti rabbit secondary nanobody fused to Tn5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human 7SK

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. phH1-gRNA

    Plasmid

    #53186

    Purpose

    Expresses the S. pyogenes sgRNA from the H1 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human H1

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-C1-Flag-SmBiT-PP1b

    Plasmid

    #224895

    Purpose

    Mammalian expression of Flag-SmBiT-PP1b

    Depositor

    Mathieu Bollen

    Article

    Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

    Insert

    Flag-SmBiT-PP1b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-HaloGFP-Na2.4

    Plasmid

    #228471

    Purpose

    Mammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

    Depositor

    Robert Campbell

    Article

    Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

    Insert

    HaloGFP-Na2.4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV GG hUbC-dsRED

    Plasmid

    #84034

    Purpose

    Contains Golden Gate cassette to express up to four gRNAs and dsRED

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p663-UBC-miniTurbo-V5-PRKACB_IDG-K

    Plasmid

    #170602

    Purpose

    Gateway destination clone of PRKACB (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-PRKACB (PRKACB Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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