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We narrowed to 7,450 results for: ef1a

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Showing: 2501 - 2550 of 7450 results

  19. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid
    #188523
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    3XNLS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188523-plasmid-map-sequence-id-372834

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  20. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid
    #188524
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    2XMTS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188524-plasmid-map-sequence-id-372835

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  39. pONSY-Lifeact:mCherry

    Plasmid
    #111876
    Purpose
    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Lifeact fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111876-plasmid-map-sequence-id-213488

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  41. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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Showing: 2501 - 2550 of 7450 results