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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmiR-report-MYB-WT

Plasmid

#141114

Purpose

Reporter plasmid of WT MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

CMYB (MYB Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmiR-report-MYB-mut

Plasmid

#141115

Purpose

Reporter plasmid of mutant MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Luciferase

Expression

Mammalian

Mutation

A to T mutations at m6A sites

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-deltaH-AMBRA1-3xFLAG

Plasmid

#174158

Purpose

Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 1-34

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_EWSR1_ERG

Plasmid

#205819

Purpose

Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

EWSR1_ERG (EWSR1, ERG Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FUS_FLI1

Plasmid

#205822

Purpose

Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

FUS_FLI1 (FUS, FLI1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

Plasmid

#223079

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH T78A

Plasmid

#212997

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

T78A

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBOBI C-FLAG PHGDH R135W

Plasmid

#212998

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

R135W

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ASCC3_GRIK2

Plasmid

#205774

Purpose

Express mEGFP-tagged fusion protein, ASCC3_GRIK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASCC3_GRIK2 (ASCC3, GRIK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NUP98_KDM5A

Plasmid

#205872

Purpose

Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_KDM5A (NUP98, KDM5A Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

Plasmid

#223087

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

Plasmid

#223094

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

Plasmid

#223075

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

Plasmid

#223068

Purpose

pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

Plasmid

#223125

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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