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pMXs-B4

Plasmid

#31708

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-92a

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
Flag FoxO3 NT

Plasmid

#14938

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO3 NT (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-249.

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Tags

HA (YDVPDYA)

Expression

Mammalian

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-SH3BP4

Plasmid

#46890

Purpose

expresses Myc tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pT2P-EF-Q7mu

Plasmid

#129303

Purpose

Positive control for ATP measurement, mitochondria outer membrane, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S11A

Plasmid

#44105

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 11 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pJZC593

Plasmid

#62319

Purpose

sgRNA with MS2-PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + MS2-PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163071

Purpose

Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDM8-GlyCAM-1/IgG

Plasmid

#17841

Depositor

Steven Rosen

Article

Bistrup et al J Cell Biol. 1999 May 17. 145(4):899-910

Insert

GlyCAM-1/IgG (Glycam1 Mouse)

Expression

Mammalian

Available Since

March 25, 2009

Availability

Academic Institutions and Nonprofits only
pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway entry vector

Tags

tdTomato

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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KA1870_pGL4-UBCmin-luc2/Esrrb-B'

Plasmid

#124196

Purpose

Luciferase reporter driven by the Esrrb enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1869_pGL4-UBCmin-luc2/Fgf4-A'

Plasmid

#124195

Purpose

Luciferase reporter driven by the Fgf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Fgf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjVASA-tdTomato

Plasmid

#160872

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

tdTomato

Use

Marmoset targeting

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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bactin:GFP-DCX

Plasmid

#105961

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only