We narrowed to 19,999 results for: IRE

Showing: 10261 - 10280 of 19999 results

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pSL0376 (His-MBP-StrepII-TniQ)

Plasmid

#130641

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite-StrepII on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pminiT-Halo-MED1 donor

Plasmid

#197868

Purpose

Donor plasmid used for building Halo-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Mouse Targeting

Tags

Halotag

Promoter

NA

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pST_DHN_AtCor47 (pBS0784)

Plasmid

#185254

Purpose

For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_AtCor47

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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cARA12-mRFP

Plasmid

#181961

Purpose

ARA12 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ARA12 (ARA12 Mustard Weed)

Tags

mRFP

Expression

Plant

Promoter

TBA2

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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LLP250 pEF1a-TALE-SunTag

Plasmid

#100939

Purpose

Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC2-UMPS (Y37A, R155A)-3xFLAG

Plasmid

#87976

Purpose

Expression of UMPS (Y37A, R155A) in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (Y37A, R155A) (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Y37A, R155A

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC2-Rap2A-3xFLAG

Plasmid

#87974

Purpose

3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_3

Plasmid

#68809

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PIWI(aa478-860)

Plasmid

#21542

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

GST

Expression

Mammalian

Mutation

contain only aa478-860

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 GAL4-fusion, Rat)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R K469R

Plasmid

#167180

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R K469R

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Txnip

Plasmid

#178437

Purpose

doxycycline-inducible expression of Mouse Txnip in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Txnip (Txnip Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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360 pCR kdrb

Plasmid

#13067

Depositor

Nathan Lawson

Article

Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

Insert

kdrb (kdr Zebrafish)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pJH2827

Plasmid

#179542

Purpose

Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAA3

Plasmid

#154163

Purpose

Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Mutation

20 wt NF-κB sites were replaced with Rel1A-specif…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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