171,009 results

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLENTI6.3/AR-GC-E2325

Plasmid

#85128

Purpose

exogenous AR expression

Depositor

Karl-Henning Kalland

Article

Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

Insert

androgen receptor (AR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-NLS-mVenus

Plasmid

#118619

Purpose

Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-NLS-mVenus

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV4.18-mut

Plasmid

#201062

Purpose

Expresses AAV2 Rep and AAV4.18 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.18 Cap

Use

AAV

Expression

Mammalian

Mutation

K492E, K503E, N585S

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pM198

Plasmid

#173218

Purpose

Expresses OptGFP(1-9)-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

OptGFP(1-9)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF3005

Plasmid

#144481

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD274

Plasmid

#232659

Purpose

transient expresses PD-L1 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD274 (CD274 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pRibo-T

Plasmid

#69343

Purpose

SQ171 fast growing strain supported by pRibo-T plasmid

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Ribo-T

Expression

Bacterial

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSAD-5PSD95-EGFP-SynPhRFP

Plasmid

#217979

Purpose

G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

5PSD95-EGFP (Dlg4 Rat)
SynPhRFP (Syp Mouse)

Use

G-deleted rabies

Tags

EGFP and RFP

Promoter

None

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Expression

Mammalian

Mutation

F23S A123V I195F

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Nme2ABE8e

Plasmid

#189928

Purpose

Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

Nme2ABE8e

Tags

NLS

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDV06.2c_DART-VADAR_iRFP720-sensor

Plasmid

#200410

Purpose

pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor detecting iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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STING-V1

Plasmid

#124262

Purpose

C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus1

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0

Plasmid

#208717

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Has Service

AAV8

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pXD68Kan2-AphA

Plasmid

#191248

Purpose

RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600E

Plasmid

#131723

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-mAmetrine1.1

Plasmid

#18084

Purpose

Bacterial expression of mAmetrine (version 1.1) fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine1.1

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only