161,273 results

1. Vimentin-pLJM5

   Plasmid

   #189868

   Purpose

   Expresses human Vimentin in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

   Insert

   Vimentin
   

   Use

   Tags

   m-Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV7 mitoDendra2

   Plasmid

   #157757

   Purpose

   Expression of the fluorescent protein Dendra2 targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MTS coxVIII +Dendra2

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYL322d1/Vn-C

   Plasmid

   #183154

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRBC-sfGFP-150TAG

   Plasmid

   #174076

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   T7

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRIPZ (M)-HT-Cbx2

   Plasmid

   #82510

   Purpose

   Expresses Halotag-Cbx2 fusion proteins in mammalian cells

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 2 (CBX2 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF1513

   Plasmid

   #143513

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX3-1 (NKX3-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-OTp-GCaMP6s

   Plasmid

   #192945

   Purpose

   To drive GCaMP6s under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRB897

    Plasmid

    #124587

    Purpose

    C-terminal fusions of C. albicans optimized mScarlet fluorescent protein.

    Depositor

    Richard Bennett

    Article

    Frazer et al Curr Protoc Microbiol. 2019 Feb 12:e76.

    Type

    Empty backbone

    Use

    Synthetic Biology; Candida albicans expression

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTOX1

    Plasmid

    #127446

    Purpose

    Encodes YhaV toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    yhaV toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. anti-Desmin_D7-pBIOCAM5

    Plasmid

    #39346

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLSSmOrange-C1

    Plasmid

    #37131

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CA-rtTA Adv

    Plasmid

    #20910

    Purpose

    piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    rtTA-ON-Adv
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-PD-1 (Y223F, Y248F)-mGFP

    Plasmid

    #180790

    Purpose

    Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1 (Y223F, Y248F) (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-Rab21-Q76L (CA)

    Plasmid

    #83422

    Purpose

    Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residue 76 from Q to L (Q76L: CAG -> CTG)

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge