169,365 results
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Plasmid#154141PurposeExpresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianMutationR887EPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxPromotervegAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
p221a-GUS
Plasmid#71263PurposeEntry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertBeta-glucuronidase
UseGatewayAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRm-NLS-dCas9-GFP11x7-NLS
Plasmid#70224PurposeExpresses NLS-dCas9-GFP11x7-NLS in mammalian cellsDepositorInsertNLS-dCas9-GFP11x7-NLS
TagsGFP11x7ExpressionMammalianAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-GCaMP8m
Plasmid#210400PurposeAAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neuronsDepositorHas ServiceAAV2InsertGCaMP8m
UseAAVExpressionMammalianPromoterPRSx8Available SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorInsertCRISPRoff-TetOn
Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-BRD4 Fusion Vector
Plasmid#237019PurposeExpress HaloTag(R)-BRD4 Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertBRD4 (BRD4 Human)
TagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable SinceOct. 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC13A5
Plasmid#131945PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC13A5 (SLC13A5 Human)
ExpressionMammalianAvailable SinceOct. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX_cpCys203
Plasmid#247756PurposeLentiviral expression of the cpCys203 FRET-based cysteine sensor in mammalian cellsDepositorInsertcpCys203 Cysteine Sensor
UseLentiviralTagsECFP and VenusExpressionMammalianAvailable SinceNov. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-LaminB
Plasmid#98830PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorInsertLaminB
TagsmTurquoise2ExpressionMammalianMutation*(see below)PromoterCMVAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGW_RUBY_EggCas9
Plasmid#225990PurposeTo clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBYDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV-RT
Plasmid#181799PurposeMCP-M-MLV-RT (MS2-N55K-MMLVRT)DepositorInsertMCP-M-MLV-RT
ExpressionMammalianAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry
Plasmid#247160PurposeEngineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3DepositorInsertPuromycin resistance, mCherry
ExpressionMammalianPromoterEf1aAvailable SinceNov. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
TagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV sTurboID (C)-Arrestin-GFP
Plasmid#153010PurposeExpresses split-TurboID C-terminal fragment fused to arrestinDepositorInsertsTurboID (C)(G74)-Arrestin
TagsGFPExpressionMammalianPromoterCMVAvailable SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3G-tdTomato
Plasmid#194644PurposeExpresses tdTomato under the control of TRE3GDepositorInsertTRE3G-tdTomato
UseAAVExpressionMammalianAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only