165,688 results

1. pcDNA5-FRT/TO-eGFP-53BP1

   Plasmid

   #60813

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Dec. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALED_Left-ND1-1397C-AD

   Plasmid

   #183892

   Purpose

   Expresses TALED_Left-ND1-1397C-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-HA (New MCS)

   Plasmid

   #32530

   Depositor

   Christopher A Walsh

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-CBE6b-IVT

   Plasmid

   #215834

   Purpose

   Template for in vitro transcription of CBE6b (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hNox4

   Plasmid

   #69352

   Purpose

   expresses human Nox4 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

   Insert

   hNOX4 (NOX4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-GFP-Syntaxin1A

   Plasmid

   #170871

   Purpose

   Mammalian expression of His-GFP-Syntaxin1A.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Syntaxin 1A (Stx1a Mouse)
   

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mKO2-N1

    Plasmid

    #54625

    Purpose

    Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Tags

    mKO2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK289 (mAID-mClover-NeoR)

    Plasmid

    #72827

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Neo
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-huMb2Cas12a

    Plasmid

    #174676

    Purpose

    Expresses 6xHis-MBP tagged Mb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5-LRRK2

    Plasmid

    #229019

    Purpose

    Expression of untagged full length human LRRK2 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    no tags (untagged)

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNL-CEF-BAFFR-CFP

    Plasmid

    #187002

    Purpose

    Expression of human BAFFR-CFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    BAFFR (TNFRSF13C Human)
    

    Use

    Lentiviral

    Tags

    CFP

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pS-sg:GFP

    Plasmid

    #196296

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid

    #174723

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and T2A linker

    Expression

    Mammalian

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRM200U

    Plasmid

    #44529

    Depositor

    Michael Grunstein

    Article

    Mann et al EMBO J. 1992 Sep;11(9):3297-306.

    Insert

    HHF2-HHT2
    

    Expression

    Yeast

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAJ075_hsCRBN

    Plasmid

    #124214

    Purpose

    Insect cell expression vector for His6 tagged hsCRBN

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    Cereblon (CRBN Human)
    

    Tags

    His6-TEV

    Expression

    Insect

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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