20,361 results

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pYPQ233 (dLbCpf1-SRDX)

Plasmid

#86211

Purpose

Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR

Tags

5' and 3' NLS and SRDX repressor

Expression

Plant

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV9:cTNT::3Flag-hYAP S127A

Plasmid

#86558

Purpose

To produce cardiac specific adeno-associated virus expressing activated human YAP.

Depositor

William Pu

Publication

Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

Insert

YAP (YAP1 Human)

Use

AAV

Tags

3Flag

Mutation

Serine 127 was mutated into Alanine, to activate …

Promoter

cTNT

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pF CAG luc puro

Plasmid

#67501

Purpose

Constitutive expression of firefly luciferase in mammalian cells

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CAG

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Publication

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Publication

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV Marina-T2A-nls-mCherry

Plasmid

#74216

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Publication

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

fluorescent protein voltage sensor

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Expression

Bacterial

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Publication

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pML104-HygMx4

Plasmid

#83475

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

Depositor

Patricia Wittkopp

Insert

HphMX4

Expression

Yeast

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pscAAV-CAG-RLuc

Plasmid

#83280

Purpose

recombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoter

Depositor

Mark Kay

Publication

Paulk et al Mol Ther Methods Clin Dev. 2018 Jul 24;1

Insert

Renilla Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPaint-TagGFP2

Plasmid

#67166

Purpose

CRISPaint universal donor for TagGFP2 fusion tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagGFP2

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_Flag_NS4A

Plasmid

#79636

Purpose

Third generation Self-inactivating lentivector expressing NS4A from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS4A

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Publication

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Publication

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLAMP1-miRFP703

Plasmid

#79998

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1 (Lamp1 Rat)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCas9-polyA

Plasmid

#72602

Purpose

express hCas9 byT7 promoter with polyadenine tails

Depositor

Tomoji Mashimo

Publication

Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-polyadenine

Tags

81-bp polyadenylation

Promoter

T7

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pG1AK-sfGFP

Plasmid

#71739

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

sfGFP

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Publication

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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