We narrowed to 12,202 results for: 112

1. pFUW-tetO-Batf3

   Plasmid

   #139837

   Purpose

   doxycycline-inducible expression of mouse Batf3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Batf3 (Batf3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

   Plasmid

   #194171

   Purpose

   Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-Associated Protein Tau 0N4R
   

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-LUC2CP/intron/ARE

   Plasmid

   #62858

   Purpose

   splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   insertion of chimeric intron at nueotide poclsiti…

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GPR87-DuET

   Plasmid

   #213293

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR87 (GPR87 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR171-DuET

   Plasmid

   #213269

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR171 (GPR171 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307 GFP-Kif26b-C

   Plasmid

   #118483

   Purpose

   lentiviral expression of GFP-Kif26b-C in mammalian cells

   Depositor

   Henry Ho

   Article

   Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

   Insert

   mouse Kif26b-C (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Encodes GFP fused to amino acid 1735 to 2112 of m…

   Promoter

   EF1A

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-CAG-IKZF3-FLAG-IRES-GFP

   Plasmid

   #107388

   Purpose

   Lentiviral expression of IKZF3-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GRM6-DuET

   Plasmid

   #213304

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM6 (GRM6 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

   Plasmid

   #139992

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FFA2-DuET

    Plasmid

    #213238

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FFA2 (FFAR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBX_RPS2_smGFP_V5

    Plasmid

    #236088

    Purpose

    Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPS2-smGFP-V5 (RPS2 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-BICD2

    Plasmid

    #164626

    Purpose

    Expresses BICD2 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    BICD2 (BICD2 Human)
    

    Tags

    Cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-IKKbeta (S177E,S181E)-pcw107

    Plasmid

    #64609

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    IKBKB
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    S177E, S181E

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. CMV-mito-GEM-GECO1

    Plasmid

    #32461

    Purpose

    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307-SARS-CoV-2-3CL

    Plasmid

    #160278

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE_hygro_mRFP1_NRF2

    Plasmid

    #136579

    Purpose

    mammalian expression of mRFP1-tagged human NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.0-TERT WT

    Plasmid

    #84924

    Purpose

    luciferase reporter for TERT promoter

    Depositor

    Joseph Costello

    Article

    Bell et al Science. 2015 May 29;348(6238):1036-9. d

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    TERT

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-GRAB_CRFmut

    Plasmid

    #208656

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGART-GFP

    Plasmid

    #99106

    Purpose

    Expresses human GART-GFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    GART (GART Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A65T, L68V, and A72S mutations in GFP gene; kanR/…

    Promoter

    CMV

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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