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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Meis1

Plasmid

#139824

Purpose

doxycycline-inducible expression of mouse Meis in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Meis1 (Meis1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI W391F V398L

Plasmid

#60787

Purpose

Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI W391F V398L

Tags

Nuclear Export Signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC-FLAG

Plasmid

#129494

Purpose

Expresses human TMEM206 tagged at the C-terminus with FLAG

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

human PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pH1R-P2A-mCherry-N1

Plasmid

#84330

Purpose

Co-expresses (untagged) Histamine-1 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R-P2A-mCherry (HRH1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

Plasmid

#171027

Purpose

Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1 and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

ChrimsonR is fused to tdTomato

Promoter

Ef1a

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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TBG-Vector

Plasmid

#109396

Purpose

AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver

Depositor

Sidi Chen

Article

Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Promoter

TBG

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM_H3.2-HaloTag

Plasmid

#244240

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H3.2 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

H3.2 (H3C14, H3C15 Human)

Use

CRISPR

Tags

HaloTag

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-EGFP

Plasmid

#127591

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPonA-BI-Gl NORM-LacZA TER- LacZB

Plasmid

#86212

Purpose

beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta-Globin (HBB, Hbb Mouse, Human)

Use

Ponasterone a inducible promoter

Tags

lacZA or lacZB

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Ponasterone A inducible

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-GCaMP6s-WPRE-pGHpA

Plasmid

#67526

Purpose

"Fluorescent reporter for calcium imaging"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

GCaMP6s

Use

AAV

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57.1 tTA SDHB

Plasmid

#177977

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

SDHB (SDHB Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Silent point mutations to SDHB sgRNA target

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Irf4

Plasmid

#139804

Purpose

doxycycline-inducible expression of mouse Irf4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf4 (Irf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-WPRE

Plasmid

#130988

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Vcp

Plasmid

#123600

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

valosin containing protein (Vcp Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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