166,201 results

1. pET21a-C2-SNAP

   Plasmid

   #208769

   Purpose

   To express C2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2-SNAP (Mfge8 Mouse)
   

   Tags

   SNAP-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. double cut NH-donor

   Plasmid

   #83576

   Purpose

   eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAH2-CageCys

   Plasmid

   #71404

   Purpose

   Expresses tRNA and synthetase for mammalian encoding of a photocaged cysteine

   Depositor

   Huiwang Ai

   Article

   Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

   Insert

   aminoacyl-tRNA synthetase for a caged cysteine
   

   Promoter

   CMV

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pRU324

   Plasmid

   #167691

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CALCRL-Tango

   Plasmid

   #66234

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CALCRL (CALCRL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

   Plasmid

   #105448

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Has Service

   AAV9

   Insert

   ChrimsonR-mRuby2-ST
   

   Use

   AAV

   Tags

   mRuby2 and soma targeting motif

   Mutation

   K176R in Chrimson

   Promoter

   hSyn

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-G protein gamma-2

   Plasmid

   #67018

   Purpose

   G protein gamma-2 tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-G protein gamma-2 (GNG2 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-CBE6a-V106W

   Plasmid

   #215824

   Purpose

   Express CBE6a-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPLp10

   Plasmid

   #170462

   Purpose

   EarlyBac insect cell expression system

   Depositor

   Hiro Furukawa

   Article

   Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

   Insert

   New vector
   

   Expression

   Insect

   Promoter

   dHsp70

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mCherry-CyrCR1

    Plasmid

    #128768

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

    Plasmid

    #37755

    Purpose

    Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    ChETA-TdTomato
    

    Use

    AAV and Cre/Lox; Cre-on

    Tags

    TdTomato

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. EFR WT

    Plasmid

    #102413

    Purpose

    fluorescent tagging. Plant expression of EFR WT

    Depositor

    Silke Robatzek

    Insert

    EFR (EFR Mustard Weed)
    

    Tags

    6xHis and mgfp5

    Expression

    Plant

    Promoter

    35S

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-CBE6a

    Plasmid

    #215819

    Purpose

    Express CBE6a (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-SpCas9-2xUGI (cas9 )
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-Ufm1 ∆C3

    Plasmid

    #133866

    Purpose

    Expression of human UFM1 mutant lacking the last three C-terminal residues

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-OTp-mCherry

    Plasmid

    #184754

    Purpose

    To drive mCherry under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

    Insert

    mCherry
    

    Use

    AAV

    Promoter

    mouse Oxytocin promoter

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW-TurboID-GOI-GFP

    Plasmid

    #209383

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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