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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Flag-His-ATM kd

Plasmid

#31986

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM kd (ATM Human)

Tags

Flag and His

Expression

Mammalian

Mutation

3'UTRf 9 nucl/D2870A N2875k. catalytically …

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCAX FLAG APP

Plasmid

#30154

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild type splice variant 751

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pUltra-hot

Plasmid

#24130

Purpose

3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

Depositor

Malcolm Moore

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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eIF2a 1

Plasmid

#21807

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry_a_tubulin_IRES_puro2

Plasmid

#21043

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pscAAV-CAG-GFP

Plasmid

#83279

Purpose

recombinant AAV vector packaging self-complementary GFP under the CAG promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

enhanced green fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-LaminA-C-18

Plasmid

#55068

Purpose

Localization: Nuclear Envelope, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Lamin A (LMNA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP ZO1

Plasmid

#30313

Depositor

Alan Fanning

Article

Fanning et al FASEB J. 2002 Nov . 16(13):1835-7.

Insert

Zonula Occludens-1 (TJP1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Unique spliceform*

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8s

Plasmid

#162371

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7

Plasmid

#136466

Purpose

Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG2(11)1-10

Plasmid

#82610

Purpose

Lentiviral vector of constitutive expression of mNeonGreen2(1-10)

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

SFFV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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