We narrowed to 21,676 results for: Tes

1. pLenti-LmoCas6-KRAB-2a-BlastR

   Plasmid

   #126492

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-KRAB-2a-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-OTUD2 (OTU, aa 132-314)

   Plasmid

   #61410

   Purpose

   Expresses human OTUD2 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-131 and aa 315-348.

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Flag-EcoCas8e

   Plasmid

   #106270

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasA
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Flag-EcoCse2

   Plasmid

   #106271

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasB
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Flag-EcoCas7

   Plasmid

   #106272

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasC
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Flag-EcoCas5

   Plasmid

   #106273

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasD
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTet-O-ATOH7-T2A-PuroR

   Plasmid

   #162343

   Purpose

   Lentiviral expression of ATOH7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   ATOH7 (ATOH7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. actc1b-mKate2-ARF1-DN

   Plasmid

   #109536

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF1 (arf1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. actc1b-mKate2-ARF6-DN

   Plasmid

   #109538

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF6 (arf6a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

    Plasmid

    #222287

    Purpose

    Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    HOOK3 (HOOK3 Human)
    

    Use

    Tags

    8 His and ZZ tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_003 sgINTS12 guide 2

    Plasmid

    #193607

    Purpose

    INTS12 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgINTS12 guide 2 (INTS12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_003 sgINTS12 guide 1

    Plasmid

    #193606

    Purpose

    INTS12 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgINTS12 guide 1 (INTS12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mKate2-DrCavin1b in pT3TS-Dest

    Plasmid

    #194295

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1b (cavin1b Zebrafish)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-DrCavin1b

    Plasmid

    #194292

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1b (cavin1b Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. actc1b-mKate2-jph1a

    Plasmid

    #109497

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to junctophilin 1a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    jph1a (jph1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-SERCA1

    Plasmid

    #109552

    Purpose

    Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry

    Plasmid

    #176016

    Purpose

    Mammalian expression of mCherry. Parton lab clone DFQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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