We narrowed to 18,089 results for: multi

1. pJK_ToeRepG2_N64_switch

   Plasmid

   #132743

   Purpose

   T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

   Depositor

   Alexander Green, Peng Yin

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   ToeRepG2_N64_switch
   

   Expression

   Bacterial

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ_Ptet*_3WJrep_N19_switch

   Plasmid

   #132739

   Purpose

   Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

   Depositor

   Alexander Green, Peng Yin

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   Ptet*_3WJrep_N19_switch
   

   Expression

   Bacterial

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ_NAND3_L11_S11_S13_S19

   Plasmid

   #132731

   Purpose

   T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

   Depositor

   Alexander Green, Peng Yin

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_NAND3_L11_S11_S13_S19
   

   Expression

   Bacterial

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-1

   Plasmid

   #103160

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-1 target
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-N-SH2

   Plasmid

   #111274

   Purpose

   express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B3200

   Plasmid

   #91079

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   none (begins with P2A to be fused to TALE-VPR_1)

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_B3400

   Plasmid

   #91080

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   none (begins with P2A to be fused to TALE-VP64_1)

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_A3101

   Plasmid

   #91048

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_A3301

   Plasmid

   #91049

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTaCas9-dead_6

    Plasmid

    #91175

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTaCas9-dead_5

    Plasmid

    #91174

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS1[GalS-L,LacI-L]

    Plasmid

    #60759

    Purpose

    Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    GalS-L
    wt LacI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with GalS L…

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pzs1[GalS-L]

    Plasmid

    #60743

    Purpose

    Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    GalS-L
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with GalS L…

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZS1[RbsR-T]

    Plasmid

    #60751

    Purpose

    Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    RbsR-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Chimeric LacI/GalR repressor with the TAN DBD and…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS1[RbsR-L]

    Plasmid

    #60741

    Purpose

    Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    RbsR-L
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with RbsR L…

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiCheck-DNAJA43'UTR

    Plasmid

    #41847

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    DNAJA4 3'UTR (DNAJA4 Human)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pPV477

    Plasmid

    #42930

    Depositor

    James Lok

    Insert

    attL1::egfp (870 bp)::attL2
    

    Use

    Multi-site gateway donor plasmid

    Available Since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC1-IMS-oROS-HT

    Plasmid

    #216419

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LSB-hsa-miR-130b-3p

    Plasmid

    #103217

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-130b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-130b-3p target (MIR130B Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-let-7a-5p

    Plasmid

    #103146

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7a-5p target
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge