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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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PHB2-GFP

Plasmid

#232788

Purpose

Encodes a GFP-tagged Prohibitin2/PHB2

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin WT

Plasmid

#16518

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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Iron Insensitive Control Construct 2

Plasmid

#243007

Purpose

Iron Insensitive Control Construct under the same expression promoter used in IronFist

Depositor

Roland Malli

Article

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Inserts

mNeonGreen
T2A
mCherry

Use

Gateway

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSOMA-NLS

Plasmid

#118937

Purpose

Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal

Expression

Plant

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-µLight

Plasmid

#201225

Purpose

Express muLight biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

µLight

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLDLR-Luc (aka: pES7)

Plasmid

#14940

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCEFL EGFP-TEADi

Plasmid

#140144

Purpose

TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Article

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
OA-1067A1

Plasmid

#164847

Purpose

Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-eGFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1067K

Plasmid

#164848

Purpose

Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-tdTomato

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OA-874PA

Plasmid

#164846

Purpose

Expresses AAEL005635-Cas9 with OpIE2-CFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

P10-3'UTR OpIE2-CFP

Expression

Insect

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUb-G76V-GFP-IRES-DsRed

Plasmid

#225503

Purpose

Expression of Ub-G76V-GFP along dsRED from one mRNA

Depositor

David Rubinsztein

Article

Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

Insert

Ub-G76V-GFP

Tags

GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTSK561

Plasmid

#190816

Purpose

For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

Depositor

Tatiana Karpova, Gunjan Mehta

Article

Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

Insert

HaloTag

Use

Bacterial cloning vector

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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