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pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

Plasmid

#71216

Purpose

dCas9 fused to human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3A catalytic domain

Use

Lentiviral

Tags

dSpCas9-DNMT3A

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_Short-IRES-eGFP

Plasmid

#196935

Purpose

Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

short isoform, lack of signaling casacade

Promoter

TRE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -2

Plasmid

#198420

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR Human CASP9 -1

Plasmid

#198419

Purpose

Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Human CASP9 Specific gRNA (CASP9 Human)

Use

CRISPR

Tags

Cas9/Orange Fluorescent Protein Reporter

Expression

Mammalian

Promoter

U6; CMV

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-WT-ATPIF1

Plasmid

#85403

Purpose

Retroviral vector expressing wild type human ATPIF1

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Wildtype

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
SARS-COV2-NSP1-HA-BASU

Plasmid

#154071

Purpose

Express SARS-CoV-2 NSP1 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/mito-mutant-hogg1

Plasmid

#18708

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

Amino acid 229, Arginine to Glutamine

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pLentiCas9-T2A-mApple

Plasmid

#193780

Purpose

Tricistronic expression of Cas9-mApple-BSD in mammalian cells

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

mApple

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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aP2-GFPtpz

Plasmid

#114211

Purpose

aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage

Depositor

David Rowe

Article

Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101

Insert

aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Aequorea victoria, Mouse, Synthetic)

Tags

None

Expression

Mammalian

Promoter

aP2/Fabp4

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Lin28

Plasmid

#60345

Purpose

Mammalian Expression, Lentiviral

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Lin28 (Lin28a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Expression

Mammalian

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS010: pLX304-CheRiff-GFP

Plasmid

#62986

Purpose

Lentivirus production of CheRiff channelrhodopsin actuator

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

CheRiff-eGFP

Use

Lentiviral; Gateway destination vector

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_LMD9

Plasmid

#110626

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3_CDC50A

Plasmid

#203694

Purpose

expresses CDC50A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX_305-N-dTAG_OGT

Plasmid

#155196

Purpose

N-terminal FKBP12(F36V)-2xHA tag on lentiviral OGT construct

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

lacking N-terminal Met

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 4-1BB]

Plasmid

#218336

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 CD28Z]

Plasmid

#218338

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

Plasmid

#154775

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAA in GFP reporter, UUA anticodon and G10A, …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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