We narrowed to 22,939 results for: REP
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Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorInsert3XP3-DsRed
UseCRISPRPromoter3XP3Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F3
Plasmid#55786PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)DepositorInsert3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
Tags3xFLAG and STREPExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1
Plasmid#39350DepositorUseYeast genomic targetingTagsnatMX6 module - reverse orientationAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA (MSW#111)
Plasmid#208066Purposeexpression of human Replication Protein A in E. coliDepositorExpressionBacterialMutationRPA32 has a point mutation Thr88(ACC) -> Ile(A…Available SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianPromoterEF-1α promoterAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKD4_mCherry
Plasmid#187386PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRTDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Rosa26-twinPE-attB
Plasmid#207859PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Rosa26-twinPE-attB
UseAAVMutationSee ManuscriptPromoterCbhAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30
Plasmid#111744PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ30 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pcDNA1 neo
Plasmid#13585DepositorInsertintegrin alpha 9 (ITGA9 Human)
ExpressionMammalianAvailable SinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_nLightR2
Plasmid#221673PurposeExpresses nLightR2 in neuronsDepositorInsertnLightR2
UseAAVExpressionMammalianPromoterhuman Synapsin-1Available SinceFeb. 26, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTW051d2
Plasmid#116893Purposehigh stringency SE-PACE APDepositorInsertsg3c
T7 RNAP C-term R632S/Q649S
Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-FlaviA-mNeptune-puro
Plasmid#140091PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-mNeptune
UseLentiviral; High expressionTagsmNeptuneExpressionMammalianPromoterCMVAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkC
Plasmid#39979DepositorInsertTrkC (Ntrk3 Rat)
TagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIR-CDH1 mutUTR
Plasmid#25039DepositorInsertCDH1 mutant 3'UTR (CDH1 Human)
UseLuciferaseExpressionMammalianMutationsubstitution of 6nt within the miR-9 binding site…Available SinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIB185
Plasmid#90196PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only