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We narrowed to 11,108 results for: HA

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Showing: 10561 - 10580 of 11108 results

  3. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid
    #114091
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R and S542R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114091-plasmid-map-sequence-id-220538

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  10. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Article
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Insert
    RNF168 (RNF168 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available Since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

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  11. hsp70: mKate2 dynactin1-811

    Plasmid
    #105970
    Purpose
    heat shock inducible transgenesis, first coiled coil domain plus some more amino acids, truncated before second coiled coil domain, it's first 811 amino acids of dynactin subunit p150 glued, has dominant negative effect on dynein function
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    dynactin1-811 (DCTN1 Human)
    Tags
    mKate2
    Expression
    Bacterial
    Mutation
    contains only amino acids 1-811
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    105970-plasmid-map-sequence-id-203781

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  13. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  14. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  15. INCTbiosyn-p35S-GFP(rc)4

    Plasmid
    #127522
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127522-plasmid-map-sequence-id-250833

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  16. Myc-gamma2S/deltaIL

    Plasmid
    #119730
    Purpose
    GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Article
    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.
    Insert
    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119730-plasmid-map-sequence-id-229624

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  17. pJTB126

    Plasmid
    #248910
    Purpose
    expresses the yeast analog-sensitive Smk1-Q120A and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Rimal et al Mol Cell Biol. 2020 Jul 29;40(16). pii:
    Inserts
    SMK1 Mitogen-activated Protein Kinase -analog-sensitive form (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes from S288C form)
    Ssp2 Activator of the Smk1 MAPK (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    codon 120 has been changed from Q to A (Smk1-Q120…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248910-plasmid-map-sequence-id-497716

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Showing: 10561 - 10580 of 11108 results