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We narrowed to 5,164 results for: pcDNA3.1+

Showing: 1561 - 1590 of 5164 results

  1. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA3.1-hRIPK1-Flag-K115R

    Plasmid
    #112488
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K115R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112488-plasmid-map-sequence-id-214538

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  11. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232338-plasmid-map-sequence-id-474537

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  13. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232339-plasmid-map-sequence-id-474533

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  15. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Use
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

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  16. pcDNA3.1-DDR1-F866Y-V5/HIS

    Plasmid
    #236009
    Purpose
    expression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung Adenocarcinoma
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    F866Y substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236009-plasmid-map-sequence-id-469590

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  17. pcDNA3.1-PDGFRA-V561D-V5/HIS

    Plasmid
    #234762
    Purpose
    Expression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activated
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Oncotarget. 2017 Nov 27;8(65):109319-109
    Insert
    PDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    V561D substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234762-plasmid-map-sequence-id-468956

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  19. pcDNA3.1-PDGFRA-R487L-V5/HIS

    Plasmid
    #234761
    Purpose
    Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    R487L substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234761-plasmid-map-sequence-id-468953

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  20. pcDNA3.1-PDGFRA-D842V-V5/HIS

    Plasmid
    #234763
    Purpose
    Expression of the D842V mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor, constantly activated
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Oncotarget. 2017 Nov 27;8(65):109319-109
    Insert
    PDGFRA-D842V receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D842V substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234763-plasmid-map-sequence-id-468952

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  22. pcDNA3.1-PDGFRA-T674I-V5/HIS

    Plasmid
    #234756
    Purpose
    Expression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinib
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    PDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    T674I substitution
    Promoter
    CMV
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    234756-plasmid-map-sequence-id-468865

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  23. YFP Rab11 Q70L in pcDNA3.1

    Plasmid
    #90467
    Purpose
    RhoA mediated cell migration
    Depositor
    Narasimhan Gautam
    Insert
    Rab11 Q70L
    Use
    Tags
    mEYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    90467-plasmid-map-sequence-id-197844

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Showing: 1561 - 1590 of 5164 results