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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,453 results for: Coli

Showing: 2601 - 2650 of 9453 results

  1. pECT3

    Plasmid
    #75450
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt3
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75450-plasmid-map-sequence-id-146478

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  2. pKB226

    Plasmid
    #170016
    Purpose
    Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Defensin HNP-1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    170016-plasmid-map-sequence-id-335960

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  10. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  12. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  14. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  18. pZH519

    Plasmid
    #102672
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102672-plasmid-map-sequence-id-199600

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  19. pZH522

    Plasmid
    #102675
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102675-plasmid-map-sequence-id-199391

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  22. pST50Trc1-DHFRHIS

    Plasmid
    #63949
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63949-plasmid-map-sequence-id-177355

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  28. pZH518

    Plasmid
    #102671
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102671-plasmid-map-sequence-id-199394

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  31. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  33. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  35. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89590-plasmid-map-sequence-id-206038

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  38. pST50Trc4-STRHISNDHFR

    Plasmid
    #64000
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64000-plasmid-map-sequence-id-177406

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  46. pST50Trc2-STRDHFR

    Plasmid
    #63955
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63955-plasmid-map-sequence-id-177361

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  47. pECM1

    Plasmid
    #75445
    Purpose
    Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt1
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75445-plasmid-map-sequence-id-146473

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  50. pST50Trc4-STRNDHFR

    Plasmid
    #63996
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63996-plasmid-map-sequence-id-177402

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Showing: 2601 - 2650 of 9453 results