We narrowed to 75,184 results for: rest
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Plasmid#160701Purposederivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sitesDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionMutationLacZ bordered by BsaI restrction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF
Plasmid#72949PurposeEntry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction siteDepositorInsertLevel 0 - ORFs
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)
Plasmid#40341DepositorInsertBlimp1 promoter (mutated) (PRDM1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationTwo AP-1 sites at -1813 and -1647 replaced with B…PromoterBlimp1Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt2(K14A)
Plasmid#86624PurposeExpresses mCherry-tagged human Akt2(K14A) membrane binding mutantDepositorInsertAkt2(K14A) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAPcrRNA_Eco
Plasmid#106276PurposeE.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
PC2328
Plasmid#232089PurposeMammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.DepositorInsertduAb with cloning Esp3I restriction site
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-NAT
Plasmid#221129PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMXDepositorInsertFAPoptim
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-CUP1pr-NFAPoptim
Plasmid#221110PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-CUP1pr-CFAPoptim
Plasmid#221111PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-NFAPoptim
Plasmid#221088PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-CFAPoptim
Plasmid#221089PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-NFAPoptim
Plasmid#221093PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPoptim
Plasmid#221094PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-ADH1pr-NFAPoptim
Plasmid#221096PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-ADH1pr-CFAPoptim
Plasmid#221097PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-ADH1pr-NFAPoptim
Plasmid#221098PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-ADH1pr-CFAPoptim
Plasmid#221099PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)+E2:E15DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-MET25pr-NFAPoptim
Plasmid#221101PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-MET25pr-CFAPoptim
Plasmid#221102PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-MET25pr-NFAPoptim
Plasmid#221104PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-MET25pr-CFAPoptim
Plasmid#221105PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-CUP1pr-NFAPoptim
Plasmid#221107PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-CUP1pr-CFAPoptim
Plasmid#221108PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLN34 - pBait-5GC[1xMS2]-McaS
Plasmid#222403Purposearabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertMcaS (mcaS E. coli)
UseTags1xMS2 hairpin, 5GC clampExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIND-GLucZ
Plasmid#228704PurposeInducible expression of Gaussia luciferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-pol2-Smarcd1-Myc
Plasmid#227716PurposeExpresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.DepositorInsertSmarcd1 (Smarcd1 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterpol2Available sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K63R)-MCS
Plasmid#212727PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K63R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo(K48R)-MCS
Plasmid#212726PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo(K48R)-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCitro-15a
Plasmid#202033Purposep15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to CitrobacterDepositorInsertconjugative system from a Citrobacter spp.
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TC9
Plasmid#202088Purposeentry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-destDepositorInsertTevCas9 + sgRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
M7C2
Plasmid#188616PurposeMinimal Conjugative Plasmid 7DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
M8C8
Plasmid#188617PurposeMinimal Conjugative Plasmid 8DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C16
Plasmid#188610PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C19
Plasmid#188611PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmBruno_N
Plasmid#147028PurposeInsect Expression of DmBrunoDepositorInsertDmBruno (aret Fly)
UseTagsExpressionInsectMutationthree non silent mutations S88T, H203R and A449R …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmBruno_N
Plasmid#147030PurposeInsect Expression of DmBrunoDepositorInsertDmBruno (aret Fly)
UseTagsExpressionInsectMutationthree non silent mutations S88T, H203R and A449R …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16-mut1
Plasmid#178906Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT miR16-5p MRE1 mutatedPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only