We narrowed to 12,945 results for: 112

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T4 Lysozyme A112D WT*

Plasmid

#18514

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

A112D WT*

Available Since

Aug. 28, 2008

Availability

Academic Institutions and Nonprofits only
pLIC.B4.SPy1128

Plasmid

#11531

Depositor

Sung-Hou Kim

Article

Xu et al Proteins. 2004 May 1. 55(2):479-81.

Insert

phosphotransacetylase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pBiT3.1-C_WDHD1:1-1129

Plasmid

#211175

Purpose

Mammalian expression of full-length human WDHD1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSN-A112C-GpA

Plasmid

#191238

Purpose

Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

Depositor

Alessandro Senes

Article

Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

Insert

nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)

Tags

nuclease A from S. aureus fused to the TM domain …

Expression

Bacterial

Mutation

Changed Alanine 112 to Cysteine

Promoter

T7 promoter

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149112)

Plasmid

#76713

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBbB8k-csg-nb112

Plasmid

#171702

Purpose

Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.

Depositor

Neel Joshi

Article

Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

Insert

csgBACEFG, with nanobody fused to CsgA

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mTcf4B (CT#1126)

Plasmid

#14019

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pBiT3.1-N_WDHD1:1-1129

Plasmid

#211134

Purpose

Mammalian expression of full-length human WDHD1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMTP112 (pMS26_miniTn6900(KanR))

Plasmid

#164260

Purpose

Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

miniTn6900 element

Use

Bacterial delivery vector for tn7 att site

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A

Plasmid

#163619

Purpose

mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

H112A

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-E112A-FL

Plasmid

#110218

Purpose

Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

E112A, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 K112E HA

Plasmid

#8952

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 K112E

Plasmid

#8951

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
CAMK2D gRNA (BRDN0001146112)

Plasmid

#77185

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2D

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2D (CAMK2D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pY112 (pcDNA3.1-huSaCpf1)

Plasmid

#92269

Purpose

Expression of humanized SaCpf1

Depositor

Feng Zhang

Insert

huSaCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL (T112A)

Plasmid

#23101

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (T112D)

Plasmid

#23102

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TD) (Bcl2l11 Mouse)

Tags

Gst

Expression

Bacterial

Mutation

Changed Threonine 112 to Aspartate

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only