We narrowed to 11,416 results for: 112

1. pY112 (pcDNA3.1-huSaCpf1)

   Plasmid

   #92269

   Purpose

   Expression of humanized SaCpf1

   Depositor

   Feng Zhang

   Insert

   huSaCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b+R3(R112C/T180C)

   Plasmid

   #108195

   Purpose

   Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C) fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. J23112-mRFP1-331Bb

   Plasmid

   #78278

   Purpose

   mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23112-mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGSTag BimEL (T112A)

   Plasmid

   #23101

   Depositor

   Roger Davis

   Article

   Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

   Insert

   Bim EL (TA) (Bcl2l11 Mouse)
   

   Use

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   Changed Threonine 112 to Alanine

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGSTag BimEL (T112D)

   Plasmid

   #23102

   Depositor

   Roger Davis

   Article

   Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

   Insert

   Bim EL (TD) (Bcl2l11 Mouse)
   

   Use

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   Changed Threonine 112 to Aspartate

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

6. Rc/CMV cyclin D2 K112E HA

   Plasmid

   #8953

   Depositor

   Philip Hinds

   Article

   Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

   Insert

   cyclin D2 (CCND2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K112E: point mutation in cyclin box

   Promoter

   Available since

   Oct. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. Salsa-BC112-P2B4

   Plasmid

   #202953

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC112

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC112
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet Response Element (TRE)

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN-A112C-1A32

   Plasmid

   #191241

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Use

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN-A112C-1A31

   Plasmid

   #191242

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Use

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSN-A112C-CP8B1

    Plasmid

    #191240

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Use

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

    Plasmid

    #185318

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

    Plasmid

    #185317

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

    Plasmid

    #185316

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid

    #105635

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid

    #105637

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

    Plasmid

    #105638

    Purpose

    Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid

    Depositor

    Thanos Halazonetis

    Inserts

    Rev7 (Mad2l2 Mouse)
    Champ1 (CHAMP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutations: F11S, G12A, V132K, C133V, A135K

    Promoter

    T7

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1C-Fluc-CG11206_A

    Plasmid

    #145900

    Purpose

    Insect Expression of CG11206-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

    Insert

    CG11206-3UTR (CG11206 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiSAMv2 rs11257655-guide1

    Plasmid

    #125460

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiSAMv2 rs11257655-guide2

    Plasmid

    #118205

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 rs11257655-guide4

    Plasmid

    #118207

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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