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pCMV-OptoFGFR1
Plasmid#59776PurposeExpresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused proteinDepositorInsertOptoFGFR1 (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NRF2
Plasmid#207811PurposeN-terminal tagged NRF2 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-NRF2 (NFE2L2 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NRF2-V5-miniTurbo
Plasmid#207812PurposeC-terminal tagged NRF2 for miniTurbo proximity experimentsDepositorInsertNRF2-V5-miniTurbo (NFE2L2 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_pBabePuro
Plasmid#54337PurposeN-terminally FLAG tagged human IRE1a in retroviral pBabe puro vectorDepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationThe endogenous IRE1a signal sequence (aa1-18) was…PromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-cAMPr
Plasmid#160723PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.DepositorInsertS2-cAMPr
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-ERK2 GOF
Plasmid#53174PurposeExpresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vectorDepositorInsertERK2 (MAPK1 Human, Mustard Weed)
UseRetroviralTagsHAExpressionMammalianMutationR67S/D321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-ExRaiAKAR
Plasmid#160724PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.DepositorInsertS3-ExRaiAKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S4-ExRaiCKAR
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 K54R
Plasmid#53170PurposeExpresses GST-HA tagged human ERK2 K54R from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human, Mustard Weed)
UseTagsGST and HAExpressionBacterialMutationK54RPromoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-ERK2 R67S
Plasmid#53172PurposeExpresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vectorDepositorInsertERK2 (MAPK1 Human, Mustard Weed)
UseRetroviralTagsHAExpressionMammalianMutationR67SPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-ERK2 D321N
Plasmid#53173PurposeExpresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vectorDepositorInsertERK2 (MAPK1 Human, Mustard Weed)
UseRetroviralTagsHAExpressionMammalianMutationD321NPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only
