We narrowed to 14,199 results for: cas9

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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)

Plasmid

#178987

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Publication

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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px458 EQR

Plasmid

#101731

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cas9-ryt

Plasmid

#246021

Purpose

Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex

Depositor

Max Scott

Inserts

Cas9
RFPex

Use

CRISPR

Tags

NLS

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

Plasmid

#69223

Purpose

Expresses R1335K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1335 is changed to Lysine

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Cas9-tra246

Plasmid

#246020

Purpose

Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

Cas9
RFPex

Use

CRISPR

Tags

NLS

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Cas9-tra135

Plasmid

#246019

Purpose

Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

Cas9
RFPex

Use

CRISPR

Tags

NLS

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Publication

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4-Cas9-V5

Plasmid

#87905

Purpose

Cloning vector

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

Cas9-V5

Use

Cloning vector

Tags

V5

Mutation

Human codon optimized

Promoter

V5

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBLO1811_arC9_noNLS_human

Plasmid

#74494

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Use

CRISPR

Tags

t2a mCherry (C-terminal on insert)

Expression

Mammalian

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX383 Lactobacillus johnsonii Cas9

Plasmid

#68320

Purpose

Lactobacillus johnsonii Cas9

Depositor

Feng Zhang

Insert

LjCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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