164,826 results

1. pORANGE GFP-Grin2a KI

   Plasmid

   #131486

   Purpose

   Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0799

   Plasmid

   #141705

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF3 (ATF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE Flag HA ADAR1 p150

   Plasmid

   #237855

   Purpose

   Lentiviral expression of ADAR1 p150

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   ADAR1 (ADAR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fragment1 with TLS2

   Plasmid

   #196983

   Purpose

   Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Intermediate fragment for assembly of gRNA-TLS2 construct
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2058

   Plasmid

   #141898

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-2 (NKX2-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Trx-mSA2

   Plasmid

   #52320

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, S tag, TEV site, and Thioredoxin

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-D3cpv

   Plasmid

   #36323

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D3cpv second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.R_TRE_minTK

   Plasmid

   #211516

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SV40 and minTK

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. KE-009

    Plasmid

    #133441

    Purpose

    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4H (EIF4H Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-SH3(5R)

    Plasmid

    #112154

    Purpose

    Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3-5R (NCK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    only the second SH3 domain is expressed in this c…

    Promoter

    CMV

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-SZT2

    Plasmid

    #87035

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

    Plasmid

    #214994

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. phCMV-229E-D15

    Plasmid

    #188908

    Purpose

    express truncated 229E spike for pseudovirus production

    Depositor

    David Nemazee

    Insert

    229E spike (S 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c-terminal 15aa deletion

    Promoter

    CMV

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mito-RA

    Plasmid

    #209866

    Purpose

    Dimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-miniTurbo

    Plasmid

    #209636

    Purpose

    Expression miniTurbo in mammalian cells

    Depositor

    Xiang Zhou

    Article

    Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

    Insert

    miniTurbo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP_Rab35 Q67L active

    Plasmid

    #47425

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 Q67L (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Q67L active

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6/V5-p53_R280K

    Plasmid

    #22933

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    R280K mutant

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

    Plasmid

    #128268

    Purpose

    Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Inserts

    MitoTrap
    FKBP(III)-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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