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-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

Plasmid

#32733

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) site CTTTGAT deletion

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
THI VP64 pGAP T-

Plasmid

#161487

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pBabe-puro

Plasmid

#19235

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Histidin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pMpGWB411

Plasmid

#68676

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQnl_tet

Plasmid

#191355

Purpose

Clostridium expression vector with TetR-repressed NanoLuc expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette

Expression

Bacterial

Promoter

PGus2A-TetO2/1

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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RBFOX3_RRM_pGEX

Plasmid

#135131

Purpose

Recombinant expression of RBFOX3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBFOX3 (RBFOX3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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HNRNPCL1_RRM_pGEX

Plasmid

#135116

Purpose

Recombinant expression of HNRNPCL1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

HNRNPCL1 (HNRNPCL1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Promoter

U6

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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PSUC2-yEGFPCLN2PEST-pRS406

Plasmid

#64405

Purpose

SUC2 promoter driven GFP expression

Depositor

Claudia Vickers

Article

Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

Insert

SUC2 promoter (SUC2 Budding Yeast)

Tags

yEGFPCLN2PEST (destabilized yeast EGFP)

Expression

Yeast

Promoter

SUC2

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-115-pBabe-puro

Plasmid

#19243

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 116 - 213 (end)

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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TAF15_RRM-ZNF_pGEX

Plasmid

#135111

Purpose

Recombinant expression of TAF15 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

TAF15 (TAF15 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET-SOMA

Plasmid

#118935

Purpose

Used to express the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pICH-chl-roGFP2-GPXC71S

Plasmid

#191703

Purpose

Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

chl-redox(ro)GFP2-GPXC71S

Use

Binary agrobacterium ti plasmid

Expression

Plant

Mutation

Peroxidatic Cysteine 71 replaced with Serine

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA-3xflagGREB1b

Plasmid

#112222

Purpose

mammalian expression of flag tagged GREB1b

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1b (GREB1 Human)

Tags

3x flag

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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