19,834 results

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pCE-BiFC-VC155

Plasmid

#22020

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VC155

Tags

HA

Expression

Worm

Mutation

Changed Alanine 206 to Lysine

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CA

Plasmid

#12523

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA Myr (PIK3CA Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele; I14…

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 WT

Plasmid

#13050

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); serine …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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YFP-GID1

Plasmid

#37305

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

See comments

Expression

Mammalian

Available Since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-eNpHR 3.0-EYFP

Plasmid

#26972

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Has Service

AAV2 and AAV5

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Nsp4-EGFP

Plasmid

#165109

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL Blast DN E-cadherin

Plasmid

#18800

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

Dom neg E-cadherin (Cdh1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

truncated form of E-cadherin (dominant negative) …

Available Since

July 15, 2008

Availability

Academic Institutions and Nonprofits only

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pUCP30T-E2Crimson

Plasmid

#78478

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

E2-Crimson

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR.CMV

Plasmid

#32688

Purpose

Mammalian expression and gateway entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Sec61B-V5-APEX2

Plasmid

#83411

Purpose

Expressed C-term tagged APEX2 on Sec61B in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

Sec61B-V5-APEX2 (SEC61B Human, Synthetic)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pTALYM3-Telo1

Plasmid

#47883

Purpose

TALE-mClover against telomere sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mClover

Use

TALEN

Tags

mClover

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60228

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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