20,084 results

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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-b2AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-Moesin-C-14

Plasmid

#55103

Purpose

Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Moesin (MSN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pJRD215

Plasmid

#167555

Purpose

Backbone plasmid to pRMA154

Depositor

Renato Morona

Article

Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

Type

Empty backbone

Use

Broad host range mobilisable

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_XBB.1.5

Plasmid

#196585

Purpose

expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

Depositor

David Nemazee

Insert

Spike (XBB.1.5)

Expression

Mammalian

Mutation

G252V, F486P on the basis of XBB

Promoter

CMV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-eGFP-N1

Plasmid

#31796

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK1

Plasmid

#78842

Purpose

To overexpress RIPK1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo

Plasmid

#127644

Purpose

Empty pLentiCRISPRv2 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Type

Empty backbone

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-syn-GCaMP6m-XC

Plasmid

#118975

Purpose

Improved genetic encoded calcium sensor based on GCaMP6m

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XC

Use

AAV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-GFP

Plasmid

#99521

Purpose

C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

GFP

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMRXIP VPS16-GFP

Plasmid

#67023

Purpose

Human VPS16 ORF was inserted into pMRXIP GFP-N vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pCAFNF-GFP

Plasmid

#13772

Purpose

Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFP

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-G

Plasmid

#127912

Purpose

a mammalian expression backbone vector for humanized mNeonGreen

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Tags

mNeonGreen

Expression

Mammalian

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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CD11b promoter in pGEM3zf(-)

Plasmid

#26168

Depositor

Daniel Tenen

Article

Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

Insert

CD11b promoter (ITGAM Human)

Expression

Bacterial

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Article

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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PGAM5

Plasmid

#38976

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PGAM5 (PGAM5 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only