165,744 results

1. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   human ChAT promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minCMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVTNT PINK1 C-myc

   Plasmid

   #13314

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-HcKCR1-EYFP

   Plasmid

   #182021

   Purpose

   Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR1
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Rab7A

   Plasmid

   #28047

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT_TO_FlagHA_USP10

   Plasmid

   #127102

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   USP10 (USP10 Human)
   

   Expression

   Mammalian

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0801

    Plasmid

    #141707

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCKO2

    Plasmid

    #125518

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-p47PX(aa2-132)

    Plasmid

    #19007

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p47phox (NCF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa2-132 (PX domains)

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pSPL

    Plasmid

    #110743

    Purpose

    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Polyhedrin and p10 promoters

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHCMV_CHIKV_E1E2

    Plasmid

    #207261

    Purpose

    Mammalian expression of Chikungunya virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GG-EBNA-OSK2M2L1-PP

    Plasmid

    #102902

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OSK2M2L1-gRNAs-PGK-puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL410_INS421

    Plasmid

    #49057

    Purpose

    Expresses luciferase under control of human insulin gene promoter

    Depositor

    Kevin Ferreri

    Article

    Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

    Insert

    human INS promoter (INS Human)
    

    Expression

    Mammalian

    Promoter

    INS promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOE_pvdJ

    Plasmid

    #110142

    Purpose

    Suicide plasmid with repair template for knockout in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

    Plasmid

    #214875

    Purpose

    Empty lentiviral backbone for guide array cloning

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Sst44-GFP

    Plasmid

    #172310

    Purpose

    SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst44
    

    Use

    AAV

    Promoter

    pB-Globin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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