159,371 results

1. pOlyA(WT)

   Plasmid

   #132391

   Purpose

   Ostreolysin A for sensing sphingomyelin/cholesterol complexes

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A wild-type
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, C94S, S151C

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pemiRFP670-tubulin

   Plasmid

   #136573

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670-tubulin
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR82-Tango

   Plasmid

   #66375

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR82 (GPR82 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. PB-TO-hNIL BSD-mApple

   Plasmid

   #182313

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stat3-C Flag pRc/CMV

   Plasmid

   #8722

   Depositor

   Jim Darnell

   Article

   Bromberg et al Cell 1999 Aug 6;98(3):295-303.

   Insert

   Stat3-C (Stat3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders…

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTec_mCherry

   Plasmid

   #229145

   Purpose

   Promoter strength evaluation of Ptec

   Depositor

   Shrikrishnan Sankaran

   Article

   Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Ptec

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 3xFlag IFIT1

   Plasmid

   #53554

   Purpose

   mammalian expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS160

   Plasmid

   #194483

   Purpose

   synDHBR Cassette

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   synDHBR Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2-QUAS:GFP-CAAX

    Plasmid

    #184813

    Purpose

    Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GFP-CAAX-pA
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    GFP-CAAX

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK19pA-MN

    Plasmid

    #86973

    Purpose

    ChIC; genomic mapping of chromatin proteins

    Depositor

    Ulrich Laemmli

    Article

    Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

    Insert

    protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1

    Plasmid

    #29676

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Mar 20. ():.

    Insert

    Pak3 (PAK3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa60-113 only; S74A F84A

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH-spsgRNA2

    Plasmid

    #64114

    Purpose

    Vector for expression of Sp sgRNA2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp sgRNA2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLminP_Luc2P_RE31

    Plasmid

    #90373

    Purpose

    Hippo - TEAD1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hippo

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOsU3

    Plasmid

    #170132

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-p18-CIBN

    Plasmid

    #188990

    Purpose

    A lentiviral plasmid encoding lysosome-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    p18

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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