159,238 results

1. pBactin-AcGFP-128-425-Akap9

   Plasmid

   #196871

   Purpose

   Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP-Akap9 (128-425) (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P2RX1-bio-His

   Plasmid

   #53359

   Purpose

   Expresses full-length P2X purinoceptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   P2RX1 (P2RX1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK_Sox10_enh_10E2_Cherry

   Plasmid

   #130590

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_10E2
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Aiolos AA130-189 in Pea

   Plasmid

   #74459

   Purpose

   Monitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)

   Depositor

   Benjamin Ebert

   Insert

   Aiolos (IKZF3) AA130-189 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHyVec13

   Plasmid

   #34796

   Depositor

   Rob Steele

   Type

   Empty backbone

   Use

   Hydra expression

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Hydra vulgaris actin promoter

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMpGWB408

   Plasmid

   #68673

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSIN-Arkadia-gRNA1

   Plasmid

   #180374

   Purpose

   targeting mouse Arkadia gene

   Depositor

   Dan Littman

   Article

   Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

   Insert

   Arkadia targeting gRNA (Rnf111 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CTPSA

   Plasmid

   #42343

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CTPSA (CTPS1 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pTK_Sox10_enh_99a_Citrine

   Plasmid

   #130589

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_99a
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTK_Pax7_enh_195_Citrine

    Plasmid

    #130604

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    Pax7_enh_195
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJIT163-AFID-3

    Plasmid

    #167359

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG8Ha-EWS-FLI1_Type2

    Plasmid

    #188042

    Purpose

    Expresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBIG1e-CDK12/Cyclin K

    Plasmid

    #231730

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBIG1e-CDK13/Cyclin K

    Plasmid

    #231731

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK13 (CDK13 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBMN-MEF2C

    Plasmid

    #232627

    Purpose

    Lentiviral plasmid expressing mouse MEF2C.

    Depositor

    Claes Wadelius

    Article

    Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

    Insert

    MEF2C (Mef2c Mouse)
    

    Use

    Lentiviral

    Tags

    HA-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-HA-RAD52-∆C

    Plasmid

    #235677

    Purpose

    Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 302-410

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid

    #233035

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

    Depositor

    Jonathan Ploski

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXJ-His-PDCD1LG2

    Plasmid

    #232660

    Purpose

    transient expresses PD-L2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    PDCD1LG2 (PDCD1LG2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AiP12311 - pAAV-AiE0226h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2311)

    Plasmid

    #230108

    Purpose

    AiE0226h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge