We narrowed to 1,407 results for: AdRed

1. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGST2-GST-TEV-OPTN S177D S473D

   Plasmid

   #187827

   Purpose

   Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   S177D S473D

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

   Plasmid

   #61935

   Purpose

   Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hRPN13 (285-407) (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Construct starts at residue 285, residues Q337 an…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #194703

   Purpose

   Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   RPS2 (RPS2 Human)
   

   Use

   Lentiviral

   Tags

   APOBEC1-HA-P2A-mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-ASAP4e-Kv-WPRE

   Plasmid

   #201031

   Purpose

   Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)
    

    Use

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 c-SRC (WT)

    Plasmid

    #42202

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    c-SRC (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L176F mutation compared to reference sequence NM_…

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    gfap

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-nuc-GsCT

    Plasmid

    #205517

    Purpose

    Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-GsCT (GNAS Human, Synthetic)
    

    Use

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-ASAP4b-Kv-WPRE

    Plasmid

    #201030

    Purpose

    Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4b-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-EF1a-MICB*005-IRES-ZsGreen

    Plasmid

    #114008

    Purpose

    Induces expression of human MICB allele 005 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICB*005 (MICB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-EF1a MICA*009-IRES-ZsGreen

    Plasmid

    #114007

    Purpose

    Induces expression of human MICA allele 009 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICA*009 (MICA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCherry-N1 GBP (GBP-mCherry)

    Plasmid

    #162879

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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