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We narrowed to 9,048 results for: sgRNA

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Showing: 1061 - 1080 of 9048 results

  12. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111427-plasmid-map-sequence-id-212460

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  16. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid
    #246438
    Purpose
    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    NmeCas9
    Nme-sgRNA
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 16 to Alanine, deleted amin…
    Promoter
    EF-1 alpha and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246438-plasmid-map-sequence-id-490930

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  17. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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  19. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  20. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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Showing: 1061 - 1080 of 9048 results