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YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K63R)-GFP

Plasmid

#212722

Purpose

Bacterial expression of His6-CUbo(K63R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K63R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo(K48R)-GFP

Plasmid

#212721

Purpose

Bacterial expression of His6-CUbo(K48R)-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo(K48R)

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(63)-His6

Plasmid

#212717

Purpose

Bacterial expression of NUbo-E3(63)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(48)-His6

Plasmid

#212714

Purpose

Bacterial expression of NUbo-E3(48)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-TEV2-Ubc7-His6

Plasmid

#212713

Purpose

Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

6xHis and MBP-TEV2

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Mutation

D387A

Promoter

UAS

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL667

Plasmid

#196815

Purpose

LrCas9 genome editing plasmid in wheat

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL668

Plasmid

#196816

Purpose

LrCas9 genome editing plasmid in larix

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

Lrk004P

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL669

Plasmid

#196817

Purpose

LrCas9 genome editing plasmid in tomato

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

SlEF1a

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL670

Plasmid

#196818

Purpose

LrCas9 cytosine base editing plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL671

Plasmid

#196819

Purpose

LrCas9 cytosine base editing plasmid in wheat

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL672

Plasmid

#196820

Purpose

LrCas9 adenine base editing plasmid V1.0 in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL673

Plasmid

#196821

Purpose

LrCas9 adenine base editing plasmid V2.0 in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB88

Plasmid

#199321

Purpose

Combine with Gal4 to direct TeNL expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::TeNL::let-858 3'UTR

Expression

Worm

Mutation

KD for calcium is 250 nM, 1 synthetic intron

Promoter

15xUAS::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB109

Plasmid

#199322

Purpose

Combine with Gal4 to direct CaMBI 300 expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::CaMBI::let-858 3'UTR

Expression

Worm

Mutation

Orange CaMBI with 300 nM KD for calcium

Promoter

15xUAS:::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Fzd1mApple

Plasmid

#203795

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

Fzd1 (fzd1 Zebrafish)

Use

Zebrafish expression

Tags

mApple

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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