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We narrowed to 13,645 results for: sequence

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Showing: 10941 - 10960 of 13645 results

  1. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid
    #111508
    Purpose
    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    VPR-dSpCas9[StaPL(TI)]
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, and S122…
    Promoter
    PGK
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111508-plasmid-map-sequence-id-214274

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  8. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid
    #184562
    Purpose
    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    NPRL2 (NPRL2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines.
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184562-plasmid-map-sequence-id-376960

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  11. pMIG-hNFATc2/C - AVITEV

    Plasmid
    #74058
    Purpose
    retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available Since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74058-plasmid-map-sequence-id-142261

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  12. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid
    #193051
    Purpose
    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    ALA7-TMD
    Tags
    Ig k-chain leader sequence, MBP, and mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193051-plasmid-map-sequence-id-386362

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  13. lentiMutB3GNT5-blast

    Plasmid
    #208380
    Purpose
    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Article
    Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0
    Insert
    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nt 922 c->g (PAM site inactivation)
    Promoter
    EF-1 alpha core promoter
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208380-plasmid-map-sequence-id-414094

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  17. Cntn2-Fc-His

    Plasmid
    #72066
    Purpose
    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Cntn2 (Cntn2 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72066-plasmid-map-sequence-id-137383

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Showing: 10941 - 10960 of 13645 results