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Showing: 10961 - 10980 of 78147 results

  1. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104907-plasmid-map-sequence-id-206042

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  2. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87828
    Purpose
    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87828-plasmid-map-sequence-id-175012

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  3. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Publication
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59314-plasmid-map-sequence-id-107094

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  4. M6PR-bio-His

    Plasmid
    #51924
    Purpose
    Expresses full-length Cation-dependent mannose-6-phosphate receptor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    M6PR (M6PR Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51924-plasmid-map-sequence-id-79906

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  7. pTrcHisA-PPARalpha(aa101-468)

    Plasmid
    #13574
    Depositor
    Martin Scanlon
    Insert
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    Tags
    x6His N-terminal, enterokinase cleavage site
    Expression
    Bacterial
    Mutation
    deleted residues 1-100.
    Available since
    March 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    13574-plasmid-map-sequence-id-142611

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  8. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    170349-plasmid-map-sequence-id-344647

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  10. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid
    #106309
    Purpose
    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.
    Depositor
    Zhe J. Liu
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p
    Insert
    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    Tags
    6xflag-3xHaloTag(MtoL)
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106309-plasmid-map-sequence-id-204684

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  18. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid
    #100281
    Purpose
    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa)
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100281-plasmid-map-sequence-id-197245

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  20. pEGFP C3-YAP-deltaC

    Plasmid
    #21126
    Depositor
    Marius Sudol
    Publication
    Tsutomu Oka & Marius et al
    Insert
    Yes-kinase associated protein (YAP1 Human)
    Tags
    EGFP-C3
    Expression
    Bacterial and Mammalian
    Mutation
    5 Carboxy-terminal amino acids -FLTWL are deleted
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
Showing: 10961 - 10980 of 78147 results