We narrowed to 2,368 results for: pcas9

1. pX330-sgRNA_Dgcr8_4

   Plasmid

   #73528

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pYPQAT06

   Plasmid

   #223378

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

   Plasmid

   #193979

   Purpose

   Expression of spCas9 and sgRNA targeting SIGIRR

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGGC-LexACasPea

   Plasmid

   #210539

   Purpose

   Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   LexA::SpCas9::PEA3AT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDL28_Cas9-His Δcys E532C/E1207C

   Plasmid

   #206289

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E1207C

   Promoter

   T7

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDL27_Cas9-His Δcys E532C/E945C

   Plasmid

   #206288

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E945C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE1176

   Plasmid

   #202774

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_tNbEU
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p8271 LentiCRISPR v2 hygro sgSIGIRR-4

    Plasmid

    #193980

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MTK3a_024

    Plasmid

    #123776

    Purpose

    Encodes spCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    spCAS9-2xNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR2KN0071

    Plasmid

    #117638

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPOR2KN0067

    Plasmid

    #117634

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR2KN0063

    Plasmid

    #117630

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPOR2KN0057

    Plasmid

    #117624

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPOR2KN0053

    Plasmid

    #117620

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPOR2KN0045

    Plasmid

    #117612

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Rpn2/Psmd1 subunit {AT2G32730}(pEPOR1CB0075)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPOR2KN0041

    Plasmid

    #117608

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_RPS4A family protein {AT5G58420 }(pEPOR1CB0071)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR2KN0032

    Plasmid

    #117604

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0027)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge