We narrowed to 7,456 results for: Lif

1. FRB-mCherry-Sec61β

   Plasmid

   #186574

   Purpose

   Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   FRB-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Halo-10-PFN1-Y6D

   Plasmid

   #204223

   Purpose

   Expression of Halo-Profilin-1(Y6D) in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Use

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Mutation

   Y6D

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-Halo-10-PFN1-R88E

   Plasmid

   #204224

   Purpose

   Expression of Halo-Profilin-1(R88E) in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Use

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Mutation

   R88E

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-EGFP barcode-1-SV40 polyA

   Plasmid

   #190865

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACS142

   Plasmid

   #204598

   Purpose

   Histone H3.3K9M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   human H3.3 K9M (H3-3A Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.3 K9M - V5

   Expression

   Mammalian

   Mutation

   H3.3 K9M

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-EGFP barcode-0-SV40 polyA

   Plasmid

   #190864

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-EGFP barcode-3-SV40 polyA

   Plasmid

   #190867

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-EGFP barcode-4-SV40 polyA

   Plasmid

   #190868

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode4
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-EGFP barcode-2-SV40 polyA

   Plasmid

   #190866

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCL1324

    Plasmid

    #217965

    Purpose

    Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Imp5 (IPO5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    plac

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-VTKD2-ChR2-mCherry

    Plasmid

    #170845

    Purpose

    AAV vector for Cre-dependent transgene expression of ChR2-mCherry in cortical interneurons under the control of the Dlx enhancer.

    Depositor

    Jordane Dimidschstein

    Article

    Liu et al Elife. 2020 Oct 9;9. pii: 55267. doi: 10

    Insert

    ChR2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dlx

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-DIO-ChR2-TdTomato

    Plasmid

    #166600

    Purpose

    Encodes Cre-dependent ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EKAR4

    Plasmid

    #174437

    Purpose

    Improved cyan/yellow FRET-based ERK kinase activity reporter.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MMLV-CAG-TVA-IRES-Puro

    Plasmid

    #172366

    Purpose

    Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + Puro
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-PD-1 G224A-mGFP

    Plasmid

    #180819

    Purpose

    Expression of human PD-1 G224A fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    G224A

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP-GFP-PI4KB

    Plasmid

    #221759

    Purpose

    Stable expression of GFP-PI4KB in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4KB (PI4KB Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS26_QUAS_CsChrimson

    Plasmid

    #175548

    Purpose

    Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

    Depositor

    Leslie Vosshall

    Article

    Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

    Insert

    CsChrimson
    

    Use

    Tags

    tdTomato

    Expression

    Insect

    Mutation

    Promoter

    QUAS

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SAC1delta452-587-FKBP-mCherry

    Plasmid

    #108131

    Purpose

    Recruitable inactive SAC1 (linker deletion)

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-451):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-451)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-DIO-APEX2NES

    Plasmid

    #79907

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    APEX2NES
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

    Plasmid

    #187925

    Purpose

    sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

    Depositor

    Martin Turner

    Article

    Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

    Insert

    hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

    Plasmid

    #166611

    Purpose

    Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2-HsProm1s1-mScarlet

    Plasmid

    #211330

    Purpose

    Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

    Plasmid

    #114369

    Purpose

    expresses Flpo recombinase-dependent GtACR2

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

    Plasmid

    #213402

    Purpose

    douple floxed, myristoylation (myr) site for membrane targeting and EGFP

    Depositor

    Katrin Willig

    Article

    Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

    Insert

    EGFP
    

    Use

    AAV

    Tags

    C-terminal (Ct) cytoplasmic domains of low densit…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CSII-IDR2-humanTRIM5-FOS

    Plasmid

    #79066

    Purpose

    Express human TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(human)-FOS (TRIM5 Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGal-BE3

    Plasmid

    #172409

    Purpose

    Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GALL

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid

    #172841

    Purpose

    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

    Plasmid

    #177182

    Purpose

    plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    pegRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p130Cas

    Plasmid

    #186131

    Purpose

    For expression of recombinant p130Cas in SF9 cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    BCAR1 (Bcar1 Rat)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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