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Showing: 2161 - 2200 of 2834 results

  1. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198218-plasmid-map-sequence-id-389373

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  6. p2Bac.His.XPD

    Plasmid
    #39217
    Depositor
    Aziz Sancar
    Insert
    XPD (ERCC2 Human)
    Tags
    His (HHYHHH)
    Expression
    Insect
    Mutation
    XPD starts at aa4
    Promoter
    Pph
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  7. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

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  8. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  9. ACOT11A

    Plasmid
    #42424
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACOT11A (ACOT11 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Available Since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15405-plasmid-map-sequence-id-203076

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  11. ACOT12BA

    Plasmid
    #42395
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACOT12BA (ACOT12 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available Since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  14. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid
    #41878
    Depositor
    Anna Huttenlocher
    Insert
    photoactivatable Rac1 (RAC1 Human)
    Use
    Zebrafish expression
    Tags
    6x His and mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available Since
    Oct. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    41878-plasmid-map-sequence-id-56671

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  15. mCherry-Sequestosome1-C-18

    Plasmid
    #55131
    Purpose
    Localization: Membrane Scaffold, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at amino acid 86 of Sequestosome1
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. mVenus-Sequestosome1-C-18

    Plasmid
    #56392
    Purpose
    Localization: Membrane Scaffold, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Sequestosome1 (SQSTM1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Starts at amino acid 86 of Sequestosome1
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pmCherry-C1-PHOSPHO1

    Plasmid
    #224896
    Purpose
    Express N-terminal mCherry fused to human PHOSPHO1 in mammalian cells
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    start codon is deleted
    Promoter
    CMV
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    224896-plasmid-map-sequence-id-456142

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  23. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Available Since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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  29. pJH136

    Plasmid
    #47554
    Purpose
    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10
    Insert
    URA3
    Use
    Pcr template
    Promoter
    Native URA3 promoter, -243 to -1.
    Available Since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    47554-plasmid-map-sequence-id-67878

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  31. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  34. pSCW02

    Plasmid
    #72301
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72301-plasmid-map-sequence-id-255198

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  39. pGBW-m4137386

    Plasmid
    #149540
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Human)
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2161 - 2200 of 2834 results