We narrowed to 1,509 results for: MB;

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pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA

Plasmid

#192159

Purpose

AAV-CRISPRa base vector

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTE4567

Plasmid

#107557

Purpose

Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(dead2)

Tags

3xHA and NLS

Expression

Mammalian

Mutation

E1080A

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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DPYS-FLAG

Plasmid

#61618

Purpose

To study the effect of DPYS activity on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidinase (DPYS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-F164L

Plasmid

#64681

Purpose

expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at F164L

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-H376Y

Plasmid

#64682

Purpose

expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at H376Y

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
AAV8-hSyn-flex-miR30-eGFP-shNts

Plasmid

#132717

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shNts (Nts Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgCIC-2

Plasmid

#74967

Purpose

sgCIC-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-PAK2 S20A

Plasmid

#31672

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 20 to Alanine

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-mTAZ-WW (86)

Plasmid

#40915

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ 14-3-3 binding site and WW domain (…

Promoter

Tac

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pmirGLO-Calb2-3'UTR

Plasmid

#68363

Purpose

Luciferase miRNA Target Expression Vector

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

Calb2 3'UTR (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

C to T change in UTR

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only
C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-TEV

Plasmid

#153296

Purpose

Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

TEV-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) 21 bp TEV recognition and cleavage site flanke…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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N-His-GvpC-ssrA

Plasmid

#153297

Purpose

Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)

Tags

His-tag and ssrA tag

Expression

Bacterial

Mutation

1) N-terminal 6xHis tag flanked by 1x glycine res…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A dn-cHSF1

Plasmid

#134724

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

None

Available Since

July 5, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pmirGlo-CALB2-3'UTR mir-30 mut2

Plasmid

#74429

Purpose

Calb2 UTR with mutated mir-30 site 1 and 2

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

CALB2 3'UTR (CALB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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