We narrowed to 10,457 results for: T7

1. pETM6-H9-mCherry

   Plasmid

   #66532

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H9

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H9

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEU3-NII-HLICNot

   Plasmid

   #140181

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   pEU3-NII-HLICNot

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a.MJ1247

   Plasmid

   #11414

   Depositor

   Sung-Hou Kim

   Article

   Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

   Insert

   3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)
   

   Use

   Tags

   T7-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_21 tRNA genes

   Plasmid

   #242430

   Purpose

   In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.

   Depositor

   Sebastian Maerkl

   Article

   Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

   Insert

   21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SMT503

   Plasmid

   #226840

   Purpose

   Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

   Depositor

   Polly Fordyce

   Article

   Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

   Insert

   Lipase A
   

   Use

   Synthetic Biology

   Tags

   SNAP and eGFP

   Expression

   Bacterial

   Mutation

   R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

   Promoter

   T7

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

   Plasmid

   #239294

   Purpose

   Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

   Depositor

   Anita Hopper

   Article

   Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

   Inserts

   T7 promoter
   ITS-tRNAIle intron
   T7 terminator - Var1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMR-A-3-Sasg

   Plasmid

   #214349

   Purpose

   Expresses T7-RNAP, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   T7-RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. small ubiquitin-like modifier 3 (SUMO3)

   Plasmid

   #221053

   Purpose

   Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   SUMO 3 (SUMO3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-2H12Matry

   Plasmid

   #221187

   Purpose

   His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen-Matry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28b-TEV-AcrIC9

    Plasmid

    #207409

    Purpose

    Protein purification vector to produce AcrIC9 in E. coli

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    T7-TEV-AcrIC9
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-3C-AcrIC8

    Plasmid

    #207410

    Purpose

    Protein purification vector to produce AcrIC8 in E. coli

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    T7-HRV3C-AcrIC8
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL28_Cas9-His Δcys E532C/E1207C

    Plasmid

    #206289

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E1207C

    Promoter

    T7

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDL27_Cas9-His Δcys E532C/E945C

    Plasmid

    #206288

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E945C

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDL26_Cas9-His Δcys M1C/E532C

    Plasmid

    #206287

    Purpose

    Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, E532C, C574S

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYUBcLIC-GFP

    Plasmid

    #183509

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET PI-SceI C-His K403A

    Plasmid

    #195612

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A1463G, A1464C

    Promoter

    T7

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28c_S(-31)-M30_dT7term

    Plasmid

    #112253

    Purpose

    Expresses the S(-31)-M30 apta-FRET RNA origami structure.

    Depositor

    Ebbe Sloth Andersen

    Article

    Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

    Insert

    S(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28c_S*5-M5_dT7term

    Plasmid

    #112254

    Purpose

    Expresses the S*5-M5 apta-FRET RNA origami structure.

    Depositor

    Ebbe Sloth Andersen

    Article

    Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

    Insert

    S*5-M5 apta-FRET RNA origami with T7 promoter and terminators.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEXP5-NT/6xHis mCherry F30 Broccoli

    Plasmid

    #169234

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-Broccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEU3-NII-GLICNot-C-BIOT

    Plasmid

    #140186

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    Biotinylation and GST

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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