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We narrowed to 1,130 results for: ncl

Showing: 1081 - 1100 of 1130 results

  1. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  2. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  6. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Use
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

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  7. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55762
    Purpose
    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    YFP(1-158)-gamma-2 (GNG2 Human, Aequorea victoria)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55762-plasmid-map-sequence-id-115200

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  10. pB-TR-hCAII

    Plasmid
    #232480
    Purpose
    Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Human carbonic anhydrase II (CA2 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232480-plasmid-map-sequence-id-471671

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  12. MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

    Plasmid
    #232411
    Purpose
    AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232411-plasmid-map-sequence-id-466142

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  13. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid
    #232408
    Purpose
    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232408-plasmid-map-sequence-id-466143

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  14. pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6

    Plasmid
    #175820
    Purpose
    pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 3 / MOS6, lacks start ATG and includes stop codon for N-terminal epitope tags
    Depositor
    Lennart Wirthmueller
    Article
    Ludke et al Plant J. 2021 Feb;105(4):994-1009. doi:
    Insert
    IMPORTIN ALPHA ISOFORM 3 (MOS6 Mustard Weed)
    Use
    Pentr plasmid for gateway cloning
    Tags
    none
    Expression
    Mutation
    Promoter
    none
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175820-plasmid-map-sequence-id-346168

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  15. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid
    #169298
    Purpose
    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    Arid3a-3'UTR-Mut (Arid3a Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169298-plasmid-map-sequence-id-337864

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  16. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid
    #169296
    Purpose
    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-Mut (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutations in miR-125b-2 and let-7c binding sites
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169296-plasmid-map-sequence-id-337837

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  17. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  18. pcDNA5 rtTA BirA eIF4G +MIC

    Plasmid
    #158790
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon)
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158790-plasmid-map-sequence-id-309812

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  19. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-CuO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232477-plasmid-map-sequence-id-471678

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Showing: 1081 - 1100 of 1130 results