We narrowed to 1,429 results for: piggyBac
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Plasmid#140022PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertPcKRS (pylS Methanosarcina bakeri)
TagsFLAGExpressionMammalianMutationM241F, A267S, Y271C, L274MPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
TagsAll FPs are fused to H2BExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9
Plasmid#84242PurposeExpresses GA-inducible KRAB-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast
Plasmid#205540PurposeExpress HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance markerDepositorInsertGag-MCP
UsePiggybacExpressionMammalianMutationWTPromoterTRE3GSAvailable SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor
Plasmid#184951PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mCherry
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-WT(21)
Plasmid#124396PurposeFull-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 (TET1 Human)
TagsFlagExpressionMammalianMutationfull length protein is 2136 aaAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-AcKRS
Plasmid#140021PurposePlasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integrationDepositorInsertAcKRS-3 (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationL301M, L305I, Y306F, L309A, C348FPromoterEF1Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102889PurposePiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CBh-dCas9-hEzh2-IRES-Hyg
Plasmid#122375PurposeExpresses dCas9-hEzh2-IRES-Hyg in mammalian cellsDepositorInsertCBh-dCas9-hEzh2-IRES-Hyg-pA
UsePiggybacExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
TagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH231-Bx-GFP-C31
Plasmid#115149PurposeEmpty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing padsDepositorInsertSafe Harbor Homology Sequences
ExpressionMammalianAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT5-d2EGFP-mCherry
Plasmid#201454PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT5_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacExpressionInsectPromoterDrosophila melanogaster U6:3Available SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6f
Plasmid#62811PurposepPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-Ric8b-Integration
Plasmid#129457PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses Ric8bDepositorAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EFS-Cas9-BlastR
Plasmid#115146PurposeSafe harbor site 231 knock-in vector with Cas9-BlastR expression cassetteDepositorInsertCas9-T2A-BlastR
UseCRISPRExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only