We narrowed to 1,376 results for: piggyBac
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Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_015
Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_043
Plasmid#123943PurposeEncodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - A1 Insulator - HygroR
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-112–858_Flag2-Blast
Plasmid#175039PurposeMammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-1–729_Flag2-Blast
Plasmid#175040PurposeMammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-∆ARD_Flag2-Blast
Plasmid#175041PurposeMammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein with deletion of the a…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL258
Plasmid#243697PurposeA piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 5' UTR replaced by the Actb 5' UTR, modified with silent mutations to allow for PCR-based analysisDepositorInsertNDUFB9 genomic locus, with its 5' UTR replaced by the ACTB 5' UTR (NDUFB9 Human)
ExpressionMammalianAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL259
Plasmid#243698PurposeA piggyBac vector containing the endogenous NDUFB9 genomic locus, with its native 3' UTR replaced by the Actb 3' UTR, modified with silent mutations to allow for PCR-based analysisDepositorInsertNDUFB9 genomic locus, with its 3' UTR replaced by the ACTB 3' UTR (NDUFB9 Human)
ExpressionMammalianAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pJL347
Plasmid#243707PurposeA piggyBac vector containing the COX5B coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.DepositorAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL308
Plasmid#243704PurposeA piggyBac vector containing the NDUFB9 coding sequence under the control of its endogenous promoter and 5' UTR, followed by the ACTB 3' UTRDepositorInsertNDUFB9 coding sequence with endogenous 5' UTR, followed by the ACTB 3' UTR (NDUFB9 Human)
ExpressionMammalianPromoterendogenous promoterAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)
Plasmid#229742PurposeExpresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR1 mutant WEVL -> AEVAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)
Plasmid#229743PurposeExpresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR2 mutant WTFI -> ATFAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-NeoCOVGT3-d2EGFP
Plasmid#201446PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT3_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP
Plasmid#201447PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP
Plasmid#201448PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only