We narrowed to 24,073 results for: Sis

Showing: 11001 - 11020 of 24073 results

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-MbPylRS

Plasmid

#217361

Purpose

Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. barkeri pyrrolysyl-tRNA synthetase

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pME minprom KalTa4

Plasmid

#195964

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter KalTA4 (Gal4)

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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UKNP1.16.3

Plasmid

#98188

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-globin_del5UTR_PTC39-xrRNA-4H

Plasmid

#108367

Purpose

Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

Depositor

Niels Gehring

Article

Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

Insert

PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites

Expression

Mammalian

Mutation

PTC39

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-LSL-nlsGFP-shRNA

Plasmid

#175584

Purpose

Conditional express shRNA with control of LoxP

Depositor

Christian Wolfrum

Article

Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pARF6(T27N)-CFP

Plasmid

#11386

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 27 to Asparagine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-SlugABE-NNG

Plasmid

#214356

Purpose

Expresses SlugABE-NNG

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG

Use

AAV

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pNoTag-DGCR8

Plasmid

#164694

Purpose

Express DGCR8 without tag in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

DGCR8 (DGCR8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR CD19-PI3K CAR

Plasmid

#113018

Purpose

for lentivirus production of CD19-PI3K CAR

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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ZapLck biosensor

Plasmid

#131584

Purpose

Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

Insert

ZapLck biosensor

Use

Lentiviral

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0112

Plasmid

#177044

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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