We narrowed to 12,781 results for: NUC

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pC1300_pUB10_pcoCASphi_E9t_MCS_version1

Plasmid

#197937

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KBB106

Plasmid

#185065

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated so C-terminal h…

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1

Plasmid

#183404

Purpose

Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

Plasmid

#183399

Purpose

Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-PAG-E2-Crimson-3XNLS-pA

Plasmid

#183801

Purpose

AAV vector to express nuclear localized E2-Crimson from PAG promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Expression

Bacterial

Promoter

Insulated ugpBp from E. coli

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-N7

Plasmid

#136102

Purpose

Nuclear targeting peptide for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSer4xAla_N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_NLS-SV40

Plasmid

#136108

Purpose

CTAG Nuclear localiation signal SV40 for fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_NLS-SV40

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEPQD0CM0705

Plasmid

#177035

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

Plasmid

#159941

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE

Plasmid

#159948

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

Plasmid

#159947

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-3XNLS-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

Plasmid

#159945

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTFP1.0-optoNES

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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